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首页> 外文期刊>Archives of Oral Biology >Role of proteoglycans on the biochemical and biomechanical properties of dentin organic matrix
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Role of proteoglycans on the biochemical and biomechanical properties of dentin organic matrix

机译：蛋白多糖对牙本质有机基质生物化学和生物力学性质的作用

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Highlights ? Removal of proteoglycans reduced the strength and altered dentin anisotropy, while depletion of GAGs decreased the energy to fracture. ? The core protein of proteoglycans may have a protective role in the biodegradation of the abundant type I collagen scaffold. ? Proteoglycans have a pivotal role in the tooth function by regulating dentin matrix mechanical behavior and biostability. Abstract Objective Proteoglycans (PGs) are multifunctional biomacromolecules of the extracellular matrix of collagen-based tissues. In teeth, besides a pivotal regulatory role on dentin biomineralization, PGs provide mechanical support to the mineralized tissue and compressive strength to the biosystem. This study assessed enzymatic protocols for selective PGs removal from demineralized dentin to determine the roles of these biomacromolecules in the bulk mechanical properties and biostability of type I collagen. Methods Selective removal of glycosaminoglycans chains (GAGs) and PGs from demineralized dentin was carried out by enzymatic digestion protocols using chondroitinase ABC (c-ABC) and trypsin (Try). A comprehensive study design included assessment of dentin matrix mass loss, biodegradability of the PGs/GAGs-depleted dentin matrix, ultimate tensile strength (UTS) and energy to fracture tests. Quantitative data was statistically analyzed by two-way and one-way ANOVA followed by the appropriate post hoc tests (α = 0.05). Results Transmission electron microscopy images show effective GAGs removal by c-ABC and Try and both enzymatic methods released statistically similar amounts of GAGs from the demineralized dentin. Try digestion resulted in about 25% dentin matrix mass loss and increased susceptibility to collagenolytic digestion when compared to c-ABC (p = 0.0224) and control (p = 0.0901). Moreover, PGs digestion by Try decreased the tensile strengths of dentin. Statistically lower energy to fracture was observed in c-ABC-treated dentin matrix. Conclusions GAGs plays a pivotal role on tissue mechanics and anisotropy, while the core protein of PGs have a protective role on matrix biostability.
机译：强调 ？去除蛋白质聚糖降低了强度和改变的牙本质各向异性，而GAG的耗尽降低了裂缝的能量。还蛋白多糖的核心蛋白在丰富的I型胶原屑骨架的生物降解中可能具有保护作用。还通过调节牙本质基质机械行为和生物稳定性，蛋白质聚糖在牙齿功能中具有枢转作用。摘要目标蛋白多糖（PGS）是胶原基组织细胞外基质的多功能生物致核细胞。在牙齿中，除了在牙本质生物蛋白化上的枢轴调节作用之外，PGS为矿化组织和对生物系统的抗压强度提供机械支撑。该研究评估了从脱矿质牙本质中去除的选择性PGS的酶促方案，以确定这些生物致摩洛族在I型胶原蛋白的体积力学性质和生物稳定性中的作用。方法采用软骨素消化方案（C-ABC）和胰蛋白酶（尝试），通过酶消化方案进行选择性去除糖胺聚糖链（GAG）和来自脱矿牙本质的PGS。综合研究设计包括牙本质基质质量损失的评估，PGS / GAG耗尽的牙本质基质的生物降解性，最终拉伸强度（UTS）和能量与裂缝试验。通过双向和单向ANOVA统计分析定量数据，然后进行适当的后HOC测试（α= 0.05）。结果透射电子显微镜图像显示C-ABC去除的有效噱头，并尝试酶法释放出从脱矿质的牙本质中释放出统计上类似的GAG。与C-ABC相比此外，通过尝试降低牙本质的拉伸强度的PGS消化。在C-ABC处理的牙本质基质中观察到统计上降低裂缝的能量。结论GAG在组织力学和各向异性上发挥着关键作用，而PG的核心蛋白对基质生物稳定性具有保护作用。

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