• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Archives of Oral Biology >Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity
【24h】

Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity

机译：胸腺vulgaris L.提取物在没有细胞毒性和遗传毒性的情况下具有抗微生物和抗炎作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Highlights ? Thymus vulgaris L. extract promoted control of biofilms of interest to oral health. ? This product showed in vitro anti-inflammatory effect in LPS-stimulated macrophages. ? The plant extract presented absence of cytotoxicity and genotoxicity. Abstract Objectives This study evaluated the biological effects of the T. vulgaris L. extract., such as antimicrobial activity on planktonic cultures and mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory activity and genotoxicity. Methods Monomicrobial biofilms of Candida albicans , Staphylococcus aureus , Enterococcus faecalis , Streptococcus mutans and Pseudomonas aeruginosa and polymicrobial biofilms composed by C. albicans with each bacterium were formed for 48 h and exposed for 5 min to the plant extract. Murine macrophages (RAW 264.7), human gingival fibroblasts (FMM-1), human breast carcinoma cells (MCF-7) and cervical carcinoma cells (HeLa) were also exposed to the plant extract for 5 min and the cell viability were analyzed by MTT, neutral red (NR) and crystal violet (CV) assays. Interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) produced by RAW 264.7 was quantified by ELISA, after 24 h exposure to the plant extract, both in the absence and presence of lipopolysaccharide (LPS) from Escherichia coli . Genotoxicity of the plant extract was evaluated by micronucleus formation (MN) in 1000 cells. The results were analyzed by T-Test or ANOVA and Tukey’s Test ( P ≤ 0.05). Results All biofilms showed significant reductions in CFU/mL (colony-forming units per milliliter). Cell viability was above 50% for all cell lines. Anti-inflammatory effect on the synthesis of IL-1β and TNF-α was observed. The MN was similar or lower than the control group in all cells. Conclusions T. vulgaris L. extract was effective against all biofilms, promoted high cell viability, anti-inflammatory effect and presented no genotoxicity.
机译：强调 ？胸腺vulgaris L.提取物促进了对口腔健康感兴趣的生物膜的控制。还该产品在LPS刺激的巨噬细胞中显示出体外抗炎作用。还植物提取物呈现不存在细胞毒性和遗传毒性。摘要目的本研究评估了vulgaris L.提取物的生物学效应。，例如对浮游培养物的抗微生物活性，例如抗微生物活性和单胞和多种细胞生物膜，细胞毒性，抗炎活性和遗传毒性。方法念珠菌白葡萄球菌，金黄色葡萄球菌，肠球菌和假霉素铜绿假单胞菌，链球菌异常和铜绿假单胞菌的单体霉素生物膜形成48小时，将5分钟暴露在植物提取物中。鼠巨噬细胞（Raw 264.7），人牙龈成纤维细胞（FMM-1），人乳腺癌细胞（MCF-7）和宫颈癌细胞（HELA）也暴露于植物提取物中5分钟，通过MTT分析细胞活力，中性红色（NR）和晶体紫（CV）测定。由Raw 264.7生产的白细胞介素-1β（IL-1β）和肿瘤坏死因子α（TNF-α）通过ELISA量化，在24小时暴露于植物提取物之后，无论是来自大胆（LPS）的脂多糖（LPS）都有吗？大肠杆菌。通过1000个细胞中的微核形成（Mn）评估植物提取物的基因毒性。通过T检验或Anova和Tukey的测试分析结果（P≤0.05）。结果所有生物膜均显示出CFU / ml（菌落形成单位）的显着降低。所有细胞系的细胞活力高于50％。观察到对IL-1β和TNF-α合成的抗炎作用。 Mn类似或低于所有细胞中的对照组。结论T.Vulgaris L.提取物对所有生物膜有效，促进了高细胞活力，抗炎作用，并没有呈遗传毒性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号