Real-time PCR-analysis of the cytochrome P450 1B1 codon 432-polymorphism.

Bruning T; Abel J; Koch B; Lorenzen K; Harth V; Donat S; Sachinidis A; Vetter H; Bolt HM; Ko Y

首页> 外文期刊>Archives of Toxicology >Real-time PCR-analysis of the cytochrome P450 1B1 codon 432-polymorphism.

【24h】

Real-time PCR-analysis of the cytochrome P450 1B1 codon 432-polymorphism.

机译：实时PCR分析细胞色素P450 1B1密码子432-多态性。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The aim of the present study was to develop and validate a rapid assay for genotyping of CYP1B1 codon 432-polymorphism. The described method is a single tube assay and combines both rapid-cycle polymerase chain reaction (PCR) with real-time monitoring by amplification and generation of the melting profiles of an allele-specific fluorescent probe. With this method 300 samples were analysed from healthy, unrelated Germans. Genotype frequency determined for the mutated allele CYP1B1*2 was 0.40. The results show that genotyping of CYP1B1 codon 432-polymorphism with a real-time fluorescence PCR method is a rapid and reliable assay for the analysis of large numbers of samples.

机译：本研究的目的是开发和验证CYP1B1密码子432-多态性的基因分型的快速测定。所描述的方法是单管测定，并通过扩增和产生等位基因特异性荧光探针的熔化谱，将快速循环聚合酶链反应（PCR）与实时监测结合。通过这种方法，从健康的无关德国人分析300个样品。针对突变的等位基因CYP1B1 * 2确定的基因型频率为0.40。结果表明，具有实时荧光PCR方法的CYP1B1密码子432-多态性的基因分型是用于分析大量样品的快速可靠的测定。

著录项

来源
《Archives of Toxicology》 |1999年第9期|共4页
作者
Bruning T; Abel J; Koch B; Lorenzen K; Harth V; Donat S; Sachinidis A; Vetter H; Bolt HM; Ko Y;
展开▼
作者单位

Institut fur Arbeitsphysiologie an der Universitat Dortmund Germany.;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类毒物学（毒理学）;
关键词
Codon; Cytochrome P-450; Polymerase Chain Reaction; Polymorphism Genetics genetics; 密码子; 细胞色素P-450; 聚合酶链反应;

机译：Codon;Cytochrome P-450;Polymerase Chain Reaction;Polymorphism Genetics genetics;密码子;细胞色素P-450;聚合酶链反应;

相似文献

外文文献
中文文献
专利

1. Real-time PCR-analysis of the cytochrome P450 1B1 codon 432-polymorphism. [J] . Bruning T, Abel J, Koch B, Archives of Toxicology . 1999,第8a9期

机译：实时PCR分析细胞色素P450 1B1密码子432-多态性。
2. Corrigendum to "Preferential induction of cytochrome P450 1A1 over cytochrome P450 1B1 in human breast epithelial cells following exposure to quercetin' [J. Steroid Biochem. Mol. Biol. 110 (2008) 157-162] [J] . Sarah M. Mense, Jalmeet Chabbra, Hari K. Bhat The Journal of Steroid Biochemistry and Molecular Biology . 2014,第Null期

机译：更正“暴露于槲皮素后人乳腺上皮细胞中细胞色素P450 1A1优先于细胞色素P450 1B1的诱导” [J. Steroid Biochem。Mol。Biol。110（2008）157-162]
3. Corrigendum to "Preferential induction of cytochrome P450 1A1 over cytochrome P450 1B1 in human breast epithelial cells following exposure to quercetin' [J. Steroid Biochem. Mol. Biol. 110 (2008) 157-162] [J] . Sarah M. Mense, Jalmeet Chabbra, Hari K. Bhat The Journal of Steroid Biochemistry and Molecular Biology . 2014,第Null期

机译：在槲皮素接触后“人乳腺上皮细胞中细胞色素上皮细胞细胞色素上皮细胞中细胞色上皮细胞中细胞色素P4501B1的优先诱导”静脉静脉。BIOL。110（2008）157-162]
4. Mass Spectrometry Identification of Membrane Interacting Peptides Suggests Membrane Topology of NADPH-cytochrome P450 oxidoreductase and Cytochrome P450 46A1 [C] . Natalia Mast, Wei-Li Liao, Irina A. Pikuleva, ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：膜相互作用肽的质谱鉴定表明NADPH-细胞色素P450氧化还原酶和细胞色素P450 46A1的膜拓扑
5. Developmental impact of cytochrome P450 1b1 and vitamin A deficiency on liver gene expression. [D] . Maguire, Meghan. 2017

机译：细胞色素P450 1b1和维生素A缺乏对肝脏基因表达的发育影响。
6. Preferential induction of cytochrome P450 1A1 over cytochrome P450 1B1 in human breast epithelial cells following exposure to quercetin [O] . Sarah M. Mense, Jaimeet Chhabra, Hari K. Bhat -1

机译：槲皮素暴露后人乳腺上皮细胞中细胞色素P450 1A1的诱导优于细胞色素P450 1B1的诱导
7. Brain Testosterone-CYP1B1 (Cytochrome P450 1B1) Generated Metabolite 6β-Hydroxytestosterone Promotes Neurogenic Hypertension and Inflammation [O] . Purnima Singh, Shubha Ranjan Dutta, Chi Young Song, 2020

机译：脑睾酮-CYP1B1（细胞色素P450 1B1）产生代谢物6β-羟基酮酮促进神经源性高血压和炎症
8. Aryl Hydrocarbon (Dioxin) Receptor/Transcription Factor and Cytochrome P450 1B1 as Targets for Breast Cancer Immunotherapy. [R] . Sherr, D. H. 2002

机译：芳基碳氢化合物（二恶英）受体/转录因子和细胞色素p450 1B1作为乳腺癌免疫治疗的目标。

Real-time PCR-analysis of the cytochrome P450 1B1 codon 432-polymorphism.

摘要

著录项

相似文献

相关主题

期刊订阅