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首页> 外文期刊>Arthritis and Rheumatism >Antibody-Secreting Cell Specificity in Labial Salivary Glands Reflects the Clinical Presentation and Serology in Patients With Sjogren's Syndrome
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Antibody-Secreting Cell Specificity in Labial Salivary Glands Reflects the Clinical Presentation and Serology in Patients With Sjogren's Syndrome

机译：唇唾液腺中的抗体分泌细胞特异性反映了Sjogren综合征患者的临床介绍和血清学

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Objective. The serologic hallmark of primary Sjogren's syndrome (SS) is the presence of IgG antibodies specific for Ro (SSA) and La (SSB). The molecular characteristics of gland-derived B cells at the site of primary SS inflammation have been described previously; however, parallels between glandular antibody-secreting cells (ASCs) and serologic antibody specificities have not been evaluated. We used recombinant monoclonal antibody (mAb) technology to study the specificities of salivary gland (SG)-derived ASCs, evaluate their molecular characteristics, and identify IgG antibody specificity. Methods. Human antibodies were generated from glandular IgG ASCs. Heavy chain and light chain use and immunoglobulin subclass were analyzed by sequencing. Enzyme-linked immunosorbent assay, indirect immunofluorescence, enzyme immunoassay, and 35S-labeled protein immunoprecipitation analysis were used to determine antibody specificity. Results. Evaluation of single ASCs in SG biopsy specimens from a patient with primary SS and a patient with SS and overlapping systemic lupus erythematosus revealed significant concordance between serum auto-antibody and glandular ASC specificities. Gland-derived ASC heavy chains and light chains were extensively somatically hypermutated, which is indicative of antigen-driven responses. Specifically, we produced the first fully human mAb derived from SGs. Conclusion. In patients with SS, the SGs are a site for the production of antibodies that extend beyond the canonical Ro and/or La SS specificities. Glandular antibody production strongly reflected the serologic humoral response in the 2 patients whom we studied.
机译：客观的。原发性Sjogren综合征（SS）的血清素标志是对RO（SSA）和LA（SSB）特异的IgG抗体存在。已经先前描述了初级Ss炎症部位的腺体衍生的B细胞的分子特性;然而，尚未评估腺抗体分泌细胞（ASCS）和血清素抗体特异性之间的平行。我们使用重组单克隆抗体（MAB）技术研究唾液腺（SG）的特异性（SG）的ASCS，评价它们的分子特征，并鉴定IgG抗体特异性。方法。人抗体由腺体IgG ascs产生。通过测序分析重链和轻链使用和免疫球蛋白亚类。使用酶联免疫吸附测定，间接免疫荧光，酶免疫测定和35s标记的蛋白质免疫沉淀分析来确定抗体特异性。结果。从初级SS和SS患者的SG活检标本中对SG活检标本的单次数评估和重叠系统性红斑狼疮在血清自身抗体和腺体ASC特异性之间显露出显着的一致性。广泛组织地高估的腺体衍生的ASC重链和轻链，这表明抗原驱动的反应。具体而言，我们生产了来自SGS的第一个完全人体mAb。结论。在SS的患者中，SGS是用于生产超出规范RO和/或LA SS特异性的抗体的部位。腺体抗体产生强烈反映了我们研究的2例患者的血清素体液反应。

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