Effect of plasma‐nitrided titanium surfaces on the differentiation of pre‐osteoblastic cells

Moura Carlos Eduardo B.; Queiroz Neto Moacir F.; Braz Janine Karla F. S.; de Medeiros Aires Michelle; Silva Farias Naisandra B.; Barboza Carlos Augusto G.; Cavalcanti Júnior Geraldo B.; Rocha Hugo Alexandre O.; Alves Junior Clodomiro

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Effect of plasma‐nitrided titanium surfaces on the differentiation of pre‐osteoblastic cells

机译：等离子体氮化钛表面对骨细胞预骨细胞分化的影响

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Abstract A titanium surface nitrided by plasma contains nitrogen ions that guarantee resistance to corrosion and biocompatibility. Despite this, no descriptions concerning the influence of the expression of cell adhesion proteins and their influence on osteogenic cell differentiation are available. Thus, the present study aimed to assess the response of murine pre‐osteoblastic cells (MC3T3‐E1) cultured on nitrided titanium surfaces. Pre‐osteoblastic cells were grown on polished titanium discs, used as controls, and on previously characterized plasma‐nitrided titanium discs. Cells from both groups were submitted to the MTT cell viability test. The expressions of α5, α2, and β1 integrin were assessed by flow cytometry and immunofluorescence, while osteocalcin expression was assessed by flow cytometry. The nitrided surface presented higher α2 and β1 integrin expressions, as well as osteocalcin expression, when compared to the polished surface, with no alterations in cell viability. These findings seem to suggest that the plasma nitriding treatment produces a titanium surface with the potential for effective in vitro osseointegration.

机译：摘要血浆氮化的钛表面含有氮离子，可保证耐腐蚀和生物相容性的耐药性。尽管如此，没有关于细胞粘附蛋白表达的影响及其对骨质发生细胞分化的影响的描述可获得。因此，本研究旨在评估在氮化钛表面培养的鼠前骨细胞（MC3T3-E1）的响应。在抛光的钛椎间盘上生长前骨细胞细胞，用作对照，并在先前表征的等离子体氮化钛盘上。两组中的细胞提交给MTT细胞活力测试。通过流式细胞术和免疫荧光评估α5，α2和β1整联蛋白的表达，而通过流式细胞术评估骨钙素表达。与抛光表面相比，氮化表面呈现较高的α2和β1整联蛋白表达，以及骨科表达，在细胞活力中没有改变。这些发现似乎表明等离子体氮化处理产生钛表面，其具有有效的体外骨整合的可能性。

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《Artificial Organs》 |2019年第8期|共9页
作者
Moura Carlos Eduardo B.; Queiroz Neto Moacir F.; Braz Janine Karla F. S.; de Medeiros Aires Michelle; Silva Farias Naisandra B.; Barboza Carlos Augusto G.; Cavalcanti Júnior Geraldo B.; Rocha Hugo Alexandre O.; Alves Junior Clodomiro;
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Department of Animal SciencesFederal Rural University of Semiarid Region (UFERSA)Mossoró Brazil;

Department of Animal SciencesFederal Rural University of Semiarid Region (UFERSA)Mossoró Brazil;

Department of Animal SciencesFederal Rural University of Semiarid Region (UFERSA)Mossoró Brazil;

Department of Animal SciencesFederal Rural University of Semiarid Region (UFERSA)Mossoró Brazil;

Department of Animal SciencesFederal Rural University of Semiarid Region (UFERSA)Mossoró Brazil;

Department of Animal SciencesFederal Rural University of Semiarid Region (UFERSA)Mossoró Brazil;

Department of Animal SciencesFederal Rural University of Semiarid Region (UFERSA)Mossoró Brazil;

Department of Animal SciencesFederal Rural University of Semiarid Region (UFERSA)Mossoró Brazil;

Department of Animal SciencesFederal Rural University of Semiarid Region (UFERSA)Mossoró Brazil;

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正文语种 eng
中图分类器官移植术;
关键词
biocompatible materials; integrins; osseointegration; plasma gases; titanium;

机译：生物相容性材料;整合;骨整合;血浆气体;钛;

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专利

1. Effect of plasma‐nitrided titanium surfaces on the differentiation of pre‐osteoblastic cells [J] . Moura Carlos Eduardo B., Queiroz Neto Moacir F., Braz Janine Karla F. S., Artificial Organs . 2019,第8期

机译：等离子体氮化钛表面对骨细胞预骨细胞分化的影响
2. The effect of plasma-nitrided titanium surfaces on osteoblastic cell adhesion, proliferation, and differentiation [J] . FerrazE.P., SaJ.C., DeOliveiraP.T., Journal of biomedical materials research, Part A . 2014,第4期

机译：等离子体氮化钛表面对成骨细胞粘附，增殖和分化的影响
3. The effect of plasma-nitrided titanium surfaces on osteoblastic cell adhesion, proliferation, and differentiation [J] . FerrazE.P., SaJ.C., DeOliveiraP.T., Journal of biomedical materials research, Part A . 2014,第4期

机译：等离子体氮化钛表面对成骨细胞粘附，增殖和分化的影响
4. RECONSTITUTED EXTRACELLULAR MATRIX IMPROVE OSTEOBLASTIC DIFFERENTIATION ONTO TITANIUM SURFACES [C] . Lia Rimondini, Federica Demarosi, Ismaela Foltran, THERMEC 2011;International conference on processing manufacturing of advanced materials . 2012

机译：重构的细胞外基质可促进成骨细胞在钛表面的分化
5. Surface modification of titanium nitride to increase cellular adhesion. [D] . Phipps, Crystal M. 2006

机译：氮化钛的表面改性可增加细胞粘附力。
6. Alkaline phosphatase levels of murine pre-osteoblastic cells on anodized and annealed titanium surfaces [O] . Sinem Yeniyol, John Lawrence Ricci 2018

机译：阳极和退火钛表面上鼠成骨细胞的碱性磷酸酶水平
7. Osteoblastic differentiation of periodontal ligament stem cells on nonstoichiometric calcium phosphate and titanium surfaces. [O] . Winning, L., Robinson, L., Boyd, A.R., 2018

机译：非化学计量磷酸钙和钛表面上牙周膜干细胞的成骨细胞分化。
8. Effect of Titanium Surface Roughness on Growth, Differentiation, and ProteinSynthesis of Cartilage and Bone Cells [R] . Martin, J. Y. 1996

机译：钛表面粗糙度对软骨和骨细胞生长，分化和蛋白质合成的影响

Effect of plasma‐nitrided titanium surfaces on the differentiation of pre‐osteoblastic cells

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