首页> 外文期刊>Australasian Plant Pathology >ElsinoA && punicae causing scab of pomegranates in South Africa does not cause disease on citrus
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ElsinoA && punicae causing scab of pomegranates in South Africa does not cause disease on citrus

机译:elsinoa& 导致南非石榴的泪滴不会导致柑橘造成疾病

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ElsinoA && punicae was shown to be responsible for a new disease on pomegranates in South Africa. This disease has been found in three of the pomegranate production areas in the Cape provinces, and is characterised by scab-like symptoms on the fruit and brown spots on the leaves. Representative isolates were characterised by morphology, cultural growth and sequencing of the ITS. Additionally, the LSU, rpb2 and TEF1-alpha gene regions were also sequenced for one representative isolate from pomegranate. Phylogenetic analyses of the four loci confirmed the isolates as E. punicae. RAPD analyses were also performed with three primers on 19 isolates of E. punicae. The analyses showed that E. punicae isolates clustered together with high support apart from the reference isolates of E. australis, E. fawcetti and E. australis pathotype jojoba. Pathogenicity tests were conducted with E. punicae and two reference isolates (E. australis, E. fawcetti - Citrus pathogens) on whole plant leaves and detached leaves of pomegranate and different citrus types [grapefruit, rough lemon, navels, Valencias and mandarins] in official quarantine facilities. The results confirmed E. punicae as a pathogen of pomegranate and not citrus.
机译:elsinoa& Punicae被证明是对南非石榴的新疾病负责。该疾病已发现在海角省份的三个石榴生产区域中,其特征在于叶子上的水果和棕色斑点的Scab样症状。代表性分离物的特征在于形态学,文化生长和其序列。另外,LSU,RPB2和TEF1-α基因区域也被测序,用于来自石榴的一个代表性分离物。四个基因座的系统发育分析证实了作为E. punicae的分离物。还在E. Pupicae的19个分离物中用三个引物进行了RAPD分析。分析表明,除了E. Australis,E. Fawcetti和E. Australis Patorotype Jojoba的参考分离株外,E.Punicae分离与高支撑物一起聚集在一起。致病性试验用大斑点和两种参考分离株(E. Australis,E. Fawcetti - Citrus病原体)进行整个植物叶子和石榴和不同的柑橘类类型[葡萄柚,粗柠檬,林,瓦伦斯和普通话]的脱离的叶子官方检疫设施。结果证实了E.Pinicae作为石榴的病原体而不是柑橘。

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