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首页> 外文期刊>International endodontic journal >Antibacterial properties of silver nanoparticles as a root canal irrigant against Enterococcus faecalis Enterococcus faecalis biofilm and infected dentinal tubules
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Antibacterial properties of silver nanoparticles as a root canal irrigant against Enterococcus faecalis Enterococcus faecalis biofilm and infected dentinal tubules

机译：银纳米粒子作为根管粪粪肠道肠球菌肠球菌的抗菌性能粪便粪动物生物膜和感染牙本质小管

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Abstract Aim To evaluate the antimicrobial action of an irrigant containing silver nanoparticles in an aqueous vehicle (AgNp), sodium hypochlorite and chlorhexidine against Enterococcus faecalis biofilm and infected dentinal tubules. Methodology Bovine dentine blocks were used for E.?faecalis biofilm development for 21?days and irrigated with 94?ppm AgNp solution, 2.5% Na OC l and 2% chlorhexidine for 5, 15 and 30?min. For infection of dentinal tubules with E.?faecalis , dentine specimens from bovine incisors were submitted to a contamination protocol over 5?days, with eight centrifugation cycles on every alternate day, and irrigated with the same solutions and time intervals used for the biofilm. The specimens were stained with the Live/Dead technique and evaluated using a confocal laser scanning microscope ( CLSM ). The bioImage_L software was used for measurement of the total biovolume of biofilm in μm 3 and percentage of viable bacteria (green cells) in biofilm and in dentinal tubules found after the irrigation. Statistical analyses were performed using Kruskal–Wallis and Dunn's tests for quantification of viable cells in biofilm, the Friedman test for comparisons of viable bacteria in dentinal tubules in different areas of the root canal and the Mann–Whitney U‐ test to compare the action of the irrigants between the two methods ( P ??0.05). Results The AgNp solution eliminated fewer bacteria, but was able to dissolve more biofilm compared with chlorhexidine ( P ??0.05). Na OC l had the greatest antimicrobial activity and biofilm dissolution capacity. AgNp solution had less antimicrobial action in infected dentinal tubules compared with Na OC l ( P ??0.05). The AgNp solution after 5?min was more effective in eliminating planktonic bacteria in dentinal tubules than in biofilm, but at 30?min fewer viable bacteria were observed in the biofilm compared with intratubular dentine ( P ??0.05). Conclusions AgNp irrigant was not as effective against E.?faecalis compared to solutions commonly used in root canal treatment. Na OC l is appropriate as an irrigant because it was effective in disrupting biofilm and in eliminating bacteria in biofilms and in dentinal tubules.
机译：摘要旨在评估含有银纳米粒子（AgNP），次氯酸钠和氯己定对肠球菌生物膜和感染牙本质小管的含银纳米粒子的抗微生物作用。方法论牛牙本质嵌段用于E.?Faecalis生物膜发育21℃，并用94℃浸泡94℃，2.5％Na OC L和2％氯己定5,15和30？min。对于用E.?Faecalis感染牙本质小管，从牛切口的牙本质标本提交到5岁以下的污染方案，每天有八个离心循环，并用相同的溶液和用于生物膜的时间间隔灌溉。用实时/死技术染色标本，并使用共聚焦激光扫描显微镜（CLSM）评估。 BioImage_L软件用于测量生物膜的μm3和生物膜中活细菌（绿色细胞）的百分比和灌溉后的牙本质小管的百分比。使用Kruskal-Wallis和Dunn的试验进行统计分析，用于定量生物膜中的活细胞，弗里德曼测试在根管和曼 - 惠特尼U-Test中的牙本质小管中的牙本质细菌中的活细菌比较，以比较动作两种方法之间的灌溉剂（p≤≤0.05）。结果AGNP溶液消除了较少的细菌，但能够与氯己定相比溶解更多的生物膜（p≤≤0.05）。 Na OC L具有最大的抗微生物活性和生物膜溶解能力。与Na OC L（P = 0.05）相比，AgNP溶液在感染的牙本质管中具有较少的抗微生物作用。 5？min后的AgNP溶液在消除牙本质小管中的浮鳞菌细菌比在生物膜中更有效，但与肠道牙本质牙本质（P = 0.05）相比，在生物膜中观察到30〜最小的活细菌。结论与常用于根管治疗的溶液相比，AGNP Insegant与E.?faecalis不如emaecalis。 Na OC L适合作为灌溉剂，因为它有效地破坏生物膜和消除生物膜和牙本质小管中的细菌。

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