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首页> 外文期刊>International immunopharmacology >Specific immune response to HBsAg is enhanced by beta-glucan oligosaccharide containing an alpha-(1-->3)-linked bond and biased towards M2/Th2.
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Specific immune response to HBsAg is enhanced by beta-glucan oligosaccharide containing an alpha-(1-->3)-linked bond and biased towards M2/Th2.

机译：通过含有α-（1-> 3） - 链接键的β-葡聚糖寡糖和偏向M2 / Th2的β-葡聚糖寡糖，对HBsAg的特异性免疫反应增强。

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    摘要

    Vaccination with Hepatitis B surface antigen (HBsAg) is being used to prevent HBV infection. The fact that 10% of vaccinees fail to develop protective antibodies has fostered a large body of research for more effective vaccination strategies. Search for new adjuvant, able to selectively trigger protective antibody production, is one of the most promising approaches. The oligosaccharide beta-(1-->6)-branched beta-(1-->3) glucohexaose is the basic unit of lentinan and several other fungal beta-glucans with immunostimulatory activity. beta-glucans stimulate innate immune response mainly through interaction with myeloid cells (macrophages) and dendritic cells. In this study, the ability of synthetic beta-(1-->6)-branched beta-(1-->3) glucohexaose analogue (beta-glu6) to enhance the immune response to HBsAg has been evaluated. Administration of synthetic beta-glu6 i.p. in BALB/c mice greatly enhanced the mobilisation and maturation of macrophages and dendritic cells to co-administered HBsAg, as compared to the antigen alone. The adjuvant effect of beta-glu6 was evident in the increase of T and B cell activation in response to HBsAg, as judged by the percentage of CD69-positive CD4(+) and CD19(+) lymphocytes in the spleen. beta-glu6 could significantly enhance the number of IL-4-producing cells in response to HBsAg, while it had no effect on the number of IFN-gamma-producing lymphocytes, suggesting a Th2 bias of the immune response. The correlate of protection for HBV vaccination, i.e. the titer of HBsAg-specific antibodies, was greatly enhanced by the use of beta-glu6 as a vaccine adjuvant. The IgG1/IgG2a ratio within the anti-HBsAg antibodies was higher in the mice immunised with HBsAg plus beta-glu6 than receiving HBsAg alone or mice administered HBsAg with Freund's adjuvant, indicating a shift towards a Th2-biased anti-inflammatory protective antibody response.
    机译：用乙型肝炎表面抗原（HBsAg）疫苗接种用于预防HBV感染。 10％的疫苗未能开发保护性抗体已经促进了更有效的疫苗接种策略的大型研究。搜索新的佐剂，能够选择性地触发保护性抗体生产，是最有前途的方法之一。寡糖β-（1 - > 6） - 血糖β-（1 - > 3）葡糖己糖是必法南的基本单位和具有免疫刺激活性的几种真菌β-葡聚糖。 β-葡聚糖主要通过与骨髓细胞（巨噬细胞）和树突细胞的相互作用刺激先天免疫应答。在该研究中，已经评估了合成β-（1 - > 6） - β-（1-> 3）葡聚糖戊糖类似物（Beta-glu6）的能力，以增强对HBsAg的免疫应答。施用合成β-glu6 i.p.在Balb / c小鼠中，与单独的抗原相比，大大提高了巨噬细胞和树突细胞的动员和成熟以共同施用HBsAg。 β-glu6的佐剂效应在响应于HBsAg的T和B细胞活化的增加而显而易见的是，通过脾脏中的CD69阳性CD4（+）和CD19（+）淋巴细胞的百分比判断。 β-Glu6可以显着提高响应HBsAg的IL-4产生细胞的数量，而它对产生IFN-Gamma的淋巴细胞的数量没有影响，表明免疫应答的Th2偏差。通过使用β-Glu6作为疫苗佐剂，大大提高了HBV疫苗接种的保护，即HBsAg特异性抗体的滴度。抗HBsAg抗体内的IgG1 / IgG2A比在用HBsAg加上β-Glu6免疫的小鼠中比单独接受HbsAg或用弗氏佐剂施用HbsAg的小鼠，表明朝向Th2-偏置的抗炎保护抗体反应的转变。

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