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首页> 外文期刊>International immunopharmacology >Chemically-modified polysaccharide extract derived from Leucaena leucocephala alters Raw 264.7 murine macrophage functions.
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Chemically-modified polysaccharide extract derived from Leucaena leucocephala alters Raw 264.7 murine macrophage functions.

机译：源自Leucaena Leucocephala的化学改性多糖提取物改变了Raw 264.7鼠巨噬细胞功能。

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摘要

In this study, a chemical modification of the polysaccharides extract (E) derived from Leucaena leucocephala seeds was performed to prepare C-glycosidic 2-propanol derivative (PE), and its sulphated derivative (SPE). This study aimed to characterize immunomodulatory activities of the original extract and its derivatives by exploring their effects on Raw macrophage 264.7 functions and their antioxidant activity. Our results indicated that PE was an effective radical scavenger to hydroxyl, peroxyl, and superoxide anion radicals, and SPE was a peroxyl radical scavenger. PE and SPE were found to influence the macrophage functions. Both of PE and SPE enhanced the macrophage proliferation and phagocytosis of FITC-zymosan; PE inhibited nitric oxide (NO) generation and tumor necrosis factor-alpha (TNF-alpha) secretion in lipopolysaccharide (LPS)-stimulated Raw macrophage 264.7. In contrast, SPE over-induced NO generation and TNF-alpha secretion. Moreover, PE strongly inhibited the binding affinity of FITC-LPS to Raw 264.7, as indicated by flow cytometry analysis. These findings revealed that PE may act as a potent anti-inflammatory agent; however SPE may act as an inducer of macrophage functions against pathogens.
机译：在该研究中，进行了从Leucaena Leucocephala种子的多糖提取物（E）的化学改性，制备C-糖苷2-丙醇衍生物（PE）及其硫酸化衍生物（SPE）。本研究旨在通过探索原料巨噬细胞264.7功能及其抗氧化活性来表征原萃取物及其衍生物的免疫调节活动。我们的结果表明，PE是对羟基，过氧基和超氧化物阴离子的有效的清除酸，并且SPE是过氧基的清除剂。发现PE和SPE影响巨噬细胞功能。 PE和SPE两者都增强了Fitc-Zymosan的巨噬细胞增殖和吞噬作用; PE抑制脂多糖（LPS）制备原料巨噬细胞264.7中的一氧化氮（NO）产生和肿瘤坏死因子-α（TNF-α）分泌物。相比之下，SPE过度诱导没有发电和TNF-α分泌。此外，PE强烈抑制FITC-LPS对原料264.7的结合亲和力，如流式细胞术分析所示。这些发现表明，PE可以作为有效的抗炎剂;然而，SPE可以作为对抗病原体的巨噬细胞功能的诱导剂。

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