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首页> 外文期刊>International Journal of Food Microbiology >Production and migration of patulin in Penicillium expansum molded apples during cold and ambient storage
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Production and migration of patulin in Penicillium expansum molded apples during cold and ambient storage

机译：冷和环境储存过程中Penicillium扩展模具苹果鉴定的生产和迁移

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摘要

The ability of three Penicillium expansum isolates to produce patulin was first evaluated in YES medium after incubation at 25 degrees C to select a high patulin producer. Then, a spore suspension of the selected P. expansum 3.78 strain was inoculated onto the surface of Golden delicious apples and incubated at 8 or 20 degrees C until the mold lesion reached a diameter of 1, 2 or 3 cm. For each lesion size, patulin was quantified from apple samples cut into 1 cm depthwise fractions and widthwise sized cylinders. Maximum patulin concentration, about 80,000 ng/g apple, was obtained at 8 degrees C for the center and surface sample of the 3 cm diameter lesion. Patulin was systematically found at the highest concentration in the lesions, but still quantified up to one centimeter next to the lesion. Patulin concentrations were not significantly different between the 8 and 20 degrees C incubation temperature, except for the 3 cm large lesions. Based on these findings, and for lesions less than or equal to 3 cm in diameter, we recommend to consumers to cut off at least 1 cm around and below the mold spot to limit patulin exposure. Apples should also be stored at cool temperatures, below 8 degrees C, to delay lesion development.
机译：在25℃温育后，首先在尤基培养基中评价三种青霉突变分离物在尤基培养基中进行评估，以选择高patulin生产商。然后，将所选的P.Aighsum 3.78菌株的孢子悬浮液接种到金色美味苹果的表面上，并在8或20℃下孵育，直到模具病变达到1,2或3cm的直径。对于每个病变尺寸，从苹果样品中量化，切成1cm深的馏分和宽度方向尺寸的圆柱体。在8℃下获得约80,000ng / g苹果的最大鉴定浓度为3cm直径的损伤的中心和表面样品。在病变中的最高浓度下系统地发现鉴定，但仍然在病变旁边定量为1厘米。除了3cm的大病变之外，Patulin浓度在8和20摄氏度之间没有显着差异。基于这些发现，对于直径小于或等于3厘米的病变，我们向消费者推荐在模具点周围和下方切断至少1厘米以限制髌粉暴露。苹果也应在低于8摄氏度的凉爽温度下储存，以延迟病变开发。

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