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首页> 外文期刊>International journal of medical microbiology: IJMM >Role for the fibrinogen-binding proteins Coagulase and Efb in the Staphylococcus aureus–Candida interaction
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Role for the fibrinogen-binding proteins Coagulase and Efb in the Staphylococcus aureus–Candida interaction

机译：纤维蛋白原结合蛋白凝固酶和EFB在金黄色葡萄球菌 - 念珠菌相互作用中的作用

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摘要

Staphylococcus aureus and Candida species are increasingly coisolated from implant-associated polymicrobial infections creating an incremental health care problem. Synergistic effects between both genera seem to facilitate the formation of mixed S. aureus–Candida biofilms, which is thought to play a critical role in coinfections with these microorganisms. To identify and characterize S. aureus factors involved in the interaction with Candida species, we affinity-panned an S. aureus phage display library against Candida biofilms in the presence or absence of fibrinogen. Repeatedly isolated clones contained DNA fragments encoding portions of the S. aureus fibrinogen-binding proteins coagulase or Efb. The coagulase binds to prothrombin in a 1:1 ratio thereby inducing a conformational change and non-proteolytic activation of prothrombin, which in turn cleaves fibrinogen to fibrin. Efb has been known to inhibit opsonization. To study the role of coagulase and Efb in the S. aureus–Candida cross-kingdom interaction, we performed flow-cytometric phagocytosis assays. Preincubation with coagulase reduced the phagocytosis of Candida yeasts by granulocytes significantly and dose-dependently. By using confocal laser scanning microscopy, we demonstrated that the coagulase mediated the formation of fibrin surrounding the candidal cells. Furthermore, the addition of Efb significantly protected the yeasts against phagocytosis by granulocytes in a dose-dependent and saturable fashion. In conclusion, the inhibition of phagocytosis of Candida cells by coagulase and Efb via two distinct mechanisms suggests that S. aureus might be beneficial for Candida to persist as it helps Candida to circumvent the host immune system.
机译：含有金黄色葡萄球菌和念珠菌物种越来越多地与植入物相关的多发性细胞感染造成增量保健问题。两种永久性之间的协同效应似乎有助于形成混合的金黄色葡萄球菌 - 念珠菌生物膜，这被认为在与这些微生物的繁殖中发挥着关键作用。识别和表征涉及念珠菌物种互动的金黄色葡萄球菌因素，我们在存在或不存在纤维蛋白原的情况下对念珠菌生物膜进行亲和力-AUREUS噬菌体展示库。反复孤立的克隆含有编码S.UUREUS纤维蛋白原结合蛋白凝固酶或EFB的部分的DNA片段。凝血酶在1：1的比例中与凝血酶一起结合，从而诱导凝血酶原的构象变化和非蛋白水解活化，这反过来切割纤维蛋白纤维蛋白。已知EFB抑制OPSON化。为了研究凝固酶和EFB在S.UUREUS-CANDIVA交叉王族相互作用中的作用，我们进行了流动细胞术吞噬菌症测定。用凝固素预孵育，显着且剂量依赖性地减少了粒细胞的念珠菌酵母的吞噬症。通过使用共聚焦激光扫描显微镜，我们证明凝固酶介导围绕候选细胞周围的纤维蛋白的形成。此外，通过剂量依赖性和可饱和的方式，添加EFB的添加显着保护抗噬菌体抗噬菌体症。总之，通过两个不同机制通过凝固酶和EFB抑制念珠菌细胞的吞噬作用表明，S.金黄色葡萄球菌可能有利于念珠菌，因为它有助于念珠菌旨在规避宿主免疫系统。

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    Carsten Fehrmann; Kerstin Jurk; Anne Bertling; Gabriela Seidel; Wolfgang Fegeler; Beate E. Kehrel; Georg Peters; Karsten Becker; Christine Heilmann;

  • 作者单位

    Institute of Medical Microbiology University Hospital of Münster D-48149 Münster Germany;

    Experimental and Clinical Haemostasis Department of Anaesthesiology and Intensive Care University Hospital of Münster D-48149 Münster Germany;

    The Interdisciplinary Center for Clinical Research (IZKF) Münster University Hospital of Münster D-48149 Münster Germany;

    Institute of Medical Microbiology University Hospital of Münster D-48149 Münster Germany;

    Institute of Medical Microbiology University Hospital of Münster D-48149 Münster Germany;

    The Interdisciplinary Center for Clinical Research (IZKF) Münster University Hospital of Münster D-48149 Münster Germany;

    Institute of Medical Microbiology University Hospital of Münster D-48149 Münster Germany;

    Institute of Medical Microbiology University Hospital of Münster D-48149 Münster Germany;

    Institute of Medical Microbiology University Hospital of Münster D-48149 Münster Germany;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 63.16;
  • 关键词

    Staphylococcus aureus; Candida sp; Mixed species biofilms; Phage display library; Coagulase; Efb;

    机译：金黄色葡萄球菌;Candida Sp;混合物种生物膜;噬菌体展示库;凝固酶;EFB;

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