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首页> 外文期刊>Advanced Science Letters >Investigation on the Photoinduced Interaction of Water Soluble Thio Glycolic Acid Capped CdSe Quantum Dots with Lysozyme
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Investigation on the Photoinduced Interaction of Water Soluble Thio Glycolic Acid Capped CdSe Quantum Dots with Lysozyme

机译:水溶性巯基乙醇酸封端的CdSe量子点与溶菌酶的光诱导相互作用研究

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摘要

Water soluble thio glycolic acid (TGA) capped CdSe quantum dots (QDs) was synthesized and its photoinduced interaction with lysozyme has been studied by absorption, steady state, time resolved and synchronous fluorescence measurements. It was found that the interaction of lysozyme occurred on the surface of CdSe QDs. The binding constant and number of binding sites were calculated from the relevant fluorescence data. Quenching follows static mechanism which was confirmed by time resolved fluorescence measurements. Conformational changes on the microenvironment of lysozyme have been studied using synchronous fluorescence measurement. Further, the influence of common ions on the binding constant has also been explored.
机译:合成了水溶性巯基乙醇酸(TGA)的CdSe量子点(QDs),并通过吸收,稳态,时间分辨和同步荧光测量研究了其与溶菌酶的光诱导相互作用。发现溶菌酶的相互作用发生在CdSe QDs表面。从相关的荧光数据计算结合常数和结合位点数。淬灭遵循静态机理,其通过时间分辨荧光测量得到证实。使用同步荧光测量研究了溶菌酶微环境的构象变化。此外,还研究了常见离子对结合常数的影响。

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