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首页> 外文期刊>International Journal of Phytoremediation >A method for screening copper-tolerant rice (Oryza sativa L.) cultivars based on hydroponic experiments and cluster analysis
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A method for screening copper-tolerant rice (Oryza sativa L.) cultivars based on hydroponic experiments and cluster analysis

机译：基于水培实验和聚类分析筛选铜耐稻（Oryza Sativa L.）品种的方法

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摘要

A method for screening copper (Cu)-tolerant rice cultivars was studied by combining hydroponic experiments and cluster analysis, and the classification of cultivars in Cu stress tolerance was done. In the first hydroponic experiment, seedlings of Jiahe991 and Xiushui114 were planted in nutrient solution with different Cu2+ concentrations from 10 to 1800 mu g/L. Results indicated that the toxic threshold of Cu concentration in solution ranged from 900 to 1200 mu g/L, since SPAD (Soil and Plant Analyzer Development, SPAD-502, a portable chlorophyll meter, Minolta Camera Co. Ltd., Japan) values of leaves and seedlings biomass of the treatments with &= 900 and/or 1200 mu g/L were significantly lower than the control. The second experiment was conducted with 16 local rice cultivars under three Cu treatments (10, 1000, and 1500 mu g/L). The 16 cultivars were well classified into tolerant, normal, and sensitive groups as a result of cluster analysis based on the relative SPAD (Soil and Plant Analyzer Development, SPAD-502, a portable chlorophyll meter, Minolta Camera Co. Ltd., Japan) value, shoot and root dry weights, root length and root dehydrogenase activity, and oxidizing capacity and shoot Cu concentration. Xiushui123, Xiushui134, Jiahe991, and Xianghu301 belonged to the tolerant group; Xiushui137 belonged to the sensitive group. The cluster analysis based on hydroponic experiments is an effective method for identifying rice cultivars that are tolerant to Cu stress. In addition, four cultivars (Xiushui123, Xiushui134, Jiahe991, and Xianghu301) are recommended in local practice.
机译：通过组合水培实验和聚类分析，研究了筛选铜（Cu） - 托尔化水稻品种的方法，并进行了Cu应力耐受性品种的分类。在第一水培实验中，Jiahe991和Xiushui114的幼苗以不同的Cu 2 +浓度从10至1800μg/ L种植。结果表明，溶液中Cu浓度的有毒阈值范围为900至1200μmg/ l，因为Spad（土壤和植物分析仪开发，Spad-502，便携式叶绿素仪表，Minolta Camers Co.Ltm，Japan）的价值叶子和幼苗生物量的处理与＆amp; = 900和/或1200 mu g / L显着低于对照。第二种实验用16个Cu处理（10,1000和1500μg/ L）的局部水稻品种进行。 16个品种以及分为宽容的，正常的，敏感人群如利用相对SPAD聚类分析的结果（土壤和植物分析发展，SPAD-502，便携式叶绿素仪，美能达相机有限公司，日本）值，芽和根系干重，根长和根脱氢酶活性，氧化能力和芽Cu浓度。 Xiushui123，Xiushui134，Jiahe991和Xianghu301属于耐受群体; Xiushui137属于敏感组。基于水培实验的聚类分析是鉴定耐水稻品种耐受Cu应力的有效方法。此外，在本地实践中建议使用四种品种（Xiushui123，Xiushui134，Jiahe991和Xianghu301）。

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  • 作者

    Lin Sen; Yang Tingting; Zhu Shaowei; Wang Jifeng; Ni Wuzhong;

  • 作者单位

    Zhejiang Univ Coll Environm &

    Resource Sci Key Lab Agr Resource &

    Environm Zhejiang Prov Hangzhou 310058 Zhejiang Peoples R China;

    Zhejiang Univ Coll Environm &

    Resource Sci Key Lab Agr Resource &

    Environm Zhejiang Prov Hangzhou 310058 Zhejiang Peoples R China;

    Zhejiang Univ Coll Environm &

    Resource Sci Key Lab Agr Resource &

    Environm Zhejiang Prov Hangzhou 310058 Zhejiang Peoples R China;

    Zhejiang Univ Coll Environm &

    Resource Sci Key Lab Agr Resource &

    Environm Zhejiang Prov Hangzhou 310058 Zhejiang Peoples R China;

    Zhejiang Univ Coll Environm &

    Resource Sci Key Lab Agr Resource &

    Environm Zhejiang Prov Hangzhou 310058 Zhejiang Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    copper tolerance; hydroponic; cluster analysis; rice (Oryza sativa L.);

    机译：铜耐受;水培;聚类分析;米（Oryza sativa L.）;

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