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首页> 外文期刊>International microbiology: the official journal of the Spanish Society for Microbiology >Targeted 16S rRNA amplicon analysis reveals the diversity of bacterial communities in carwash effluents
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Targeted 16S rRNA amplicon analysis reveals the diversity of bacterial communities in carwash effluents

机译：靶向16s的RRNA扩增子分析揭示了洗车流出物中的细菌群落的多样性

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摘要

This study aimed to analyze the bacterial diversity in carwash effluents and to determine their potential for use in microbial degradation of environmental contaminants. Nine carwash effluent samples were collected for physicochemical and bacterial community diversity analysis using multi-digital probes and 16S rRNA gene amplicon sequencing respectively. The pH of all effluent samples was neutral to slightly alkaline. Oil and grease concentrations ranged from 15.3 to 49.7 mg/L. 16S gene amplicon sequencing of the nine samples produced 45,934-sequence reads, which translated to 13 bacterial phyla, 26 classes, and 43 genera. The most dominant phyla were Proteobacteria, Bacteroidetes, Firmicutes, and Fusobacteria. Canonical correspondence analysis (CCA) showed that the distribution of the phyla Proteobacteria, Bacteroidetes, Acidobacteria, and Verrucomicrobia was influenced by the presence of oil and grease, total petroleum hydrocarbons-gasoline range organics (GRO-TPH), and metals species (Pb, Cu, and Zn). The dominant bacterial genera found in the present study were previously proven to biodegrade hydrocarbons, and their presence in carwash effluents could bode well for in situ natural bioremediation of these contaminated sites.
机译：本研究旨在分析洗车流出物中的细菌多样性，并确定其在环境污染物微生物降解中使用的潜力。收集九个洗车流出物样品，用于使用多数字探针和16S rRNA基因扩增子测序分别用于物理化学和细菌群落分集分析。所有流出物样品的pH中性为略微碱性。油和油脂浓度范围为15.3至49.7 mg / L. 16S基因扩增子测序九个样品的测序产生了45,934序列读数，其转化为13个细菌植物，26级和43属。最占优势的植物是植物，菌斑，骨髓，骨骼和Fusobacteria。规范对应分析（CCA）表明，Phyla植物植物，嗜血杆菌，抗酸杆菌和疣状病症的分布受油和油脂的存在，总石油烃 - 汽油范围有机（Gro-TPH）和金属物种（Pb， Cu和Zn）。先前已被证明在本研究中发现的显性细菌属于生物降解的烃，它们在洗车流出物中的存在可以很好地为这些受污染部位的原位天然生物修复。

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