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首页> 外文期刊>BJU international >The growth inhibitory effect of p21 adenovirus on androgen-dependent and -independent human prostate cancer cells.
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The growth inhibitory effect of p21 adenovirus on androgen-dependent and -independent human prostate cancer cells.

机译：p21腺病毒对雄激素依赖性和非依赖性人类前列腺癌细胞的生长抑制作用。

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摘要

OBJECTIVE: To assess the potential of p21 as a gene therapy treatment for prostate cancer, by introducing p21 into both androgen-dependent (AD) and -independent (AI) human prostate cancer cell lines via a recombinant adenoviral vector, Ad5CMV-p21, carrying human p21 cDNA. MATERIALS AND METHODS: The LNCaP, DU145 and PC-3 human prostate cancer cell lines were cultured and infected with Ad5CMV-p21. Cell growth, cell-cycle progression and tumorigenicity were then assessed by thymidine incorporation into cellular DNA, and cell number, flow cytometry, and tumour growth after inoculating the cells into nude mice. RESULTS: Growth was inhibited in Ad5CMV-p21 viral-infected AD and AI prostate cancer cells. The effects were dose-dependent, regardless of the androgen status of the cell lines. Flow cytometric analysis showed that Ad5CMV-p21 arrested cell-cycle progression at G1/S with no appreciable effect on the levels of apoptotic cells. The tumorigenicity of cancer cells infected with Ad5CMV-p21 was greatly reduced in athymic mice. CONCLUSIONS: These results suggest that Ad5CMV-p21 may be a new therapeutic agent for human prostate cancer gene therapy.
机译：目的：通过重组腺病毒载体Ad5CMV-p21将p21引入雄激素依赖性（AD）和非依赖性（AI）人前列腺癌细胞系中，评估p21作为前列腺癌基因治疗方法的潜力人p21 cDNA。材料与方法：培养LNCaP，DU145和PC-3人前列腺癌细胞系并用Ad5CMV-p21感染。然后通过将胸腺嘧啶脱氧核苷掺入细胞DNA中，然后将细胞接种到裸鼠中，评估细胞生长，细胞周期进程和致瘤性，并评估细胞数量，流式细胞仪和肿瘤生长。结果：Ad5CMV-p21病毒感染的AD和AI前列腺癌细胞的生长受到抑制。无论细胞系的雄激素状态如何，其作用都是剂量依赖性的。流式细胞仪分析表明，Ad5CMV-p21在G1 / S处阻止了细胞周期的进程，对凋亡细胞的水平没有明显影响。在无胸腺小鼠中，Ad5CMV-p21感染的癌细胞的致瘤性大大降低。结论：这些结果表明，Ad5CMV-p21可能是一种新的人前列腺癌基因治疗药物。

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