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首页> 外文期刊>BJU international >Kit-positive interstitial cells in the rabbit urethra: structural relationships with nerves and smooth muscle.
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Kit-positive interstitial cells in the rabbit urethra: structural relationships with nerves and smooth muscle.

机译：兔尿道中的kit阳性间质细胞：与神经和平滑肌的结构关系。

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OBJECTIVE: To identify interstitial cells (ICs) in the wall of the rabbit urethra using antibodies to the Kit receptor, and to examine their location, morphology and relationship with nerves and smooth muscle cells (SMCs), as studies of enzymatically isolated cells from the rabbit urethra have established that there are specialized cells that show spontaneous electrical activity and have morphological properties of ICs. MATERIALS AND METHODS: Urethral tissues from rabbits were fixed, labelled with antibodies and examined with confocal microscopy. Some specimens were embedded in paraffin wax and processed for histology. Histological sections from the most proximal third and mid-third region of rabbit urethra were stained with Masson's Trichrome to show their cellular arrangement. RESULTS: Sections from both regions had outer longitudinal and inner circular layers of SM, and a lamina propria containing connective tissue and blood vessels; the lumen was lined with urothelial cells. The mid-third region had a more developed circular SM layer than the most-proximal samples, and had extensive inner longitudinal SM bundles in the lamina propria. Labelling with anti-Kit revealed immunopositive cells within the wall of the rabbit urethra, in the circular and longitudinal layers of the muscularis. Double-labelling with an antibody to SM myosin showed Kit-positive cells on the boundary of the SM bundles, orientated parallel to the axis of the bundles. Others were in spaces between the bundles and often made contact with each other. Kit-positive cells were either elongated, with several lateral branches, or stellate with branches coming from a central soma. Similar cells could be labelled with vimentin antibodies. Their relationship with intramural nerves was examined by double immunostaining with an anti-neurofilament antibody. There were frequent points of contact between Kit-positive cells and nerves, with similar findings in specimens double-immunostained with anti-neuronal nitric oxide synthase (nNOS). CONCLUSION: Kit-positive ICs were found within the SM layers of the rabbit urethra, in association with nerves, on the edge of SM bundles and in the interbundle spaces. The contact with nNOS-containing neurones might imply participation in the nitrergic inhibitory neurotransmission of the urethra.
机译：目的：使用Kit受体抗体鉴定兔尿道壁间质细胞（IC），并检查它们的位置，形态以及与神经和平滑肌细胞（SMCs）的关系，作为对这些动物的酶促分离细胞的研究。兔尿道已确定存在显示自发电活动并具有IC形态学特性的专门细胞。材料与方法：固定兔的尿道组织，用抗体标记并用共聚焦显微镜检查。一些标本包埋在石蜡中并进行组织学处理。用Masson's Trichrome对兔尿道最近的第三和中部区域的组织学切片进行染色，以显示其细胞排列。结果：两个区域的切片均具有SM的纵向外层和内部圆形层，以及包含结缔组织和血管的固有层。管腔内衬有尿路上皮细胞。与最接近的样本相比，中三分之一区域的圆形SM层更发达，并且固有层中具有广泛的内部纵向SM束。用anti-Kit进行标记后，可在兔尿道壁内，肌层的圆形和纵向层中发现免疫阳性细胞。用针对SM肌球蛋白的抗体进行的双重标记显示，SM细胞束边界上的Kit阳性细胞平行于细胞束的轴取向。其他的则位于束之间的空间中，并且经常彼此接触。试剂盒阳性细胞或者是伸长的，带有几个侧向分支，或者是星状的，其分支来自中央躯体。相似的细胞可以用波形蛋白抗体标记。通过与抗神经丝抗体的双重免疫染色检查了它们与壁内神经的关系。 Kit阳性细胞与神经之间经常有接触点，在用抗神经元一氧化氮合酶（nNOS）双重免疫染色的标本中也有类似的发现。结论：在兔尿道SM层内，与神经相关，SM束边缘和束间间隙中均发现了试剂盒阳性IC。与含nNOS的神经元接触可能暗示参与尿道的硝化抑制性神经传递。

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