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Novel Multiplexed Biosensor System for the Determination of Lactate and Pyruvate in Blood Serum

机译：用于测定血液血清乳酸乳酸和丙酮酸的新型多重生物传感器系统

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摘要

Multiplexing is one of the main current trends in biosensors, especially important for clinical diagnosis. However, simultaneous determination of several substances in one sample is often difficult due to different performance and working conditions of separate biosensors. This work was aimed at the development of a multiplexed biosensor system for the determination of lactate and pyruvate concentrations in liquid samples (i. e., blood serum). The system consisted of two amperometric biosensors based on lactate oxidase and pyruvate oxidase, which worked simultaneously in a single measuring cell. Conditions for the biosensor system work were selected. Linear range of lactate determination was 5-1000 mu M, pyruvate - 10-5000 mu M. Steady-state response time was 30 s and 50 s for the lactate and pyruvate biosensors, respectively. After 2 weeks of storage biosensor responses decreased to 95 % (lactate biosensor) or 82 % (pyruvate biosensor) of the initial value. A scheme of analysis of the concentrations of lactate and pyruvate in human blood serum was proposed. The lactate and pyruvate concentrations as well as their ratio in human blood serum samples were determined and compared with the control method. The proposed biosensor system is suitable for the rapid detection of lactate, pyruvate and their ratio and can be used for clinical diagnosis, e. g., evaluation of the reasons of lactic acidosis and prognosis of patient's recovery.
机译：多路复用是生物传感器的主要电流趋势之一，对临床诊断尤为重要。然而，由于单独的生物传感器的不同性能和工作条件，同时测定一个样品中的几种物质通常很困难。这项工作旨在开发用于测定液体样品中的乳酸和丙酮酸浓度的多路复用生物传感器系统（即，血液血清）。该系统由基于乳酸氧化酶和丙酮酸氧化酶的两种水平体传感器组成，其在单个测量细胞中同时工作。选择了生物传感器系统工作的条件。线性范围的乳酸乳酸测定为5-1000 mu m，丙酮酸 - 10-5000 mu m.稳态响应时间分别为30 s和50s，分别用于乳酸和丙酮酸生物传感器。经过2周的储存生物传感器反应降至95％（乳酸生物传感器）或初始值的82％（丙酮酸生物传感器）。提出了一种分析人血清中乳酸浓度和丙酮酸血清浓度的方案。测定乳酸和丙酮酸浓度以及它们在人血清样品中的比例并与对照方法进行比较。所提出的生物传感器系统适用于快速检测乳酸，丙酮酸盐及其比例，可用于临床诊断，E。 G.，评价乳酸酸中毒的原因及患者恢复预后。

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