Graphene Oxide Modified Chemically Activated Graphite Electrodes for Detection of microRNA

Erdem Arzum; Eksin Ece; Isin Deniz; Polat Derya

摘要

Abstract >In our study, graphene oxide (GO) modified graphite electrodes were used for sensitive and selective impedimetric detection of miRNA. After chemical activation of pencil graphite electrode (PGE) surface using covalent agents (CA), GO modification was performed at the surface of chemically activated PGE. Then, CA‐GO‐PGEs were applied for impedimetric miRNA detection. The microscopic and electrochemical characterization of CA‐GO‐PGEs was performed by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The optimization of experimental conditions; such as GO concentration, DNA probe concentration and miRNA target concentration was performed by using EIS technique. After the hybridization occurred between miRNA‐34a RNA target and its complementary DNA probe, the hybrid was immobilized onto the surface of CA‐GO‐PGEs. Then, the impedimetric detection of miRNA‐DNA hybridization was performed by EIS. The selectivity of our assay was also tested under the optimum experimental conditions. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >研究，石墨烯（GO）改性石墨电极用于MiRNA的敏感和选择性阻碍检测。在使用共价试剂（CA）的铅笔石墨电极（PGE）表面的化学活化之后，在化学活化的PGE的表面上进行GO改性。然后，施用CA-Go-PGES用于阻抗miRNA检测。通过扫描电子显微镜（SEM）和电化学阻抗光谱（EIS）进行CA-Go-PGES的显微镜和电化学表征。实验条件的优化;例如，通过使用EIS技术进行DNA探针浓度和miRNA靶浓度。在miRNA-34A RNA靶标和其互补DNA探针之间发生杂交后，将杂交物固定在Ca-Go-PGES的表面上。然后，通过EIS进行MiRNA-DNA杂交的阻抗检测。我们的测定选择性也在最佳的实验条件下进行测试。</ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22546/'>《Electroanalysis》</a> <b style="margin: 0 2px;">|</b><span>2017年第5期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Erdem Arzum&option=202" target="_blank" rel="nofollow">Erdem Arzum;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Eksin Ece&option=202" target="_blank" rel="nofollow">Eksin Ece;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Isin Deniz&option=202" target="_blank" rel="nofollow">Isin Deniz;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Polat Derya&option=202" target="_blank" rel="nofollow">Polat Derya;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Faculty of Pharmacy Analytical Chemistry DepartmentEge University35100 Bornova Izmir TURKEY;</p> <p>Faculty of Pharmacy Analytical Chemistry DepartmentEge University35100 Bornova Izmir TURKEY;</p> <p>Faculty of Pharmacy Analytical Chemistry DepartmentEge University35100 Bornova Izmir TURKEY;</p> <p>Faculty of Pharmacy Analytical Chemistry DepartmentEge University35100 Bornova Izmir TURKEY;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4817.html" title="电化学、电解、磁化学">电化学、电解、磁化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Graphene oxide&option=203" rel="nofollow">Graphene oxide;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=miRNA&option=203" rel="nofollow">miRNA;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=nucleic acid hybridization&option=203" rel="nofollow">nucleic acid hybridization;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=pencil graphite electrode&option=203" rel="nofollow">pencil graphite electrode;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=electrochemical impedance spectroscopy.&option=203" rel="nofollow">electrochemical impedance spectroscopy.;</a> </p> <div class="translation"> 机译：石墨烯;miRNA;核酸杂交;铅笔石墨电极;电化学阻抗光谱。; 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