Separation/Concentration‐signal‐amplification in‐One Method Based on Electrochemical Conversion of Magnetic Nanoparticles for Electrochemical Biosensing

Cao Lulu; Zhang Qi; Dai Huang; Fu Yingchun; Li Yanbin

摘要

Abstract > We propose a separation/concentration‐signal‐amplification in‐one method based on electrochemical conversion (ECC) of magnetic nanoparticles (MNPs) to develop a facile and sensitive electrochemical biosensor for chloramphenicol (CAP) detection. Briefly, aptamer‐modified magnetic nanoparticles (MNPs‐Apt) was designed to capture CAP in sample, then the MNPs‐Apt composite was conjugated to Au electrode through the DNA hybridization between the unoccupied aptamer and a strand of complementary DNA. The ECC method was applied to transfer MNPs labels to electrochemically active Prussian blue (PB). The anodic and cathodic currents of PB were taken for signal readout. Comparing with conventional methods that require electrochemically active labels and related sophisticated labelling procedures, this method explored and integrated the magnetic and electrochemical properties of MNPs into one system, in turn realized magnetic capturing of CAP and signal generation without any additional conventional labels. Taking advantages of the high abundance of iron content in MNPs and the refreshing effect deriving from ECC process, the method significantly promoted the signal amplification. Therefore, the proposed biosensors exhibited linear detection range from 1 to 1000?ng?mL ?1 and a limit of detection down to 1?ng?mL ?1 , which was better than or comparable with those of most analogues, as well as satisfactory specificity, storage stability and feasibility for real samples. The developed method may lead to new concept for rapid and facile biosensing in food safety, clinic diagnose/therapy and environmental monitoring fields. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >我们提出了一种基于电化学转换的分离/浓度信号放大 - ECC）磁性纳米颗粒（MNP），用于开发用于氯霉素（帽）检测的容易和敏感的电化学生物传感器。简而言之，设计适体改性的磁性纳米颗粒（MNPS-APT）以捕获样品中的盖子，然后通过未占用的适体和一股互补DNA之间的DNA杂交将MNPS-APT复合物与Au电极缀合。应用ECC方法将MNP标签转移到电化学活性普鲁士蓝（PB）中。 PB的阳极和阴极电流被用于信号读数。与需要电化学活性标签和相关复杂标记程序的常规方法进行比较，该方法探索并将MNP的磁性和电化学性能集成到一个系统中，反过来实现了帽和信号发电的磁捕获，而无需任何额外的传统标签。采用MNP中的高丰度的铁含量和从ECC工艺中得出的清爽效果的优点，方法显着促进了信号放大。因此，所提出的生物传感器表现出直线检测范围从1至1000？ng？ml Δ1</ sup>和检测极限下降至1？ng？ml ？1 </ sup>与大多数类似物的那些更好或比较，以及真实样本的令人满意的特异性，储存稳定性和可行性。开发的方法可能导致食品安全，临床诊断/治疗和环境监测领域的快速和容易生物溶解的新概念。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22546/'>《Electroanalysis》</a> <b style="margin: 0 2px;">|</b><span>2018年第3期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cao Lulu&option=202" target="_blank" rel="nofollow">Cao Lulu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Qi&option=202" target="_blank" rel="nofollow">Zhang Qi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dai Huang&option=202" target="_blank" rel="nofollow">Dai Huang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fu Yingchun&option=202" target="_blank" rel="nofollow">Fu Yingchun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Yanbin&option=202" target="_blank" rel="nofollow">Li Yanbin;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>College of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhou 310058 China;</p> <p>College of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhou 310058 China;</p> <p>College of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhou 310058 China;</p> <p>College of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhou 310058 China;</p> <p>College of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhou 310058 China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4817.html" title="电化学、电解、磁化学">电化学、电解、磁化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Magnetic nanoparticles&option=203" rel="nofollow">Magnetic nanoparticles;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=electrochemical conversion&option=203" rel="nofollow">electrochemical conversion;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=signal amplification&option=203" rel="nofollow">signal amplification;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=antibiotics&option=203" rel="nofollow">antibiotics;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=electrochemical biosensor&option=203" rel="nofollow">electrochemical biosensor;</a> </p> <div class="translation"> 机译：磁性纳米粒子;电化学转化;信号放大;抗生素;电化学生物传感器; 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