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首页> 外文期刊>Epigenetics: official journal of the DNA Methylation Society >Comparison of DNA methylation measured by Illumina 450K and EPIC BeadChips in blood of newborns and 14-year-old children
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Comparison of DNA methylation measured by Illumina 450K and EPIC BeadChips in blood of newborns and 14-year-old children

机译：Illumina 450K测量的DNA甲基化与新生儿和14岁儿童血液中的史诗珠替

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Analysis of DNA methylation helps to understand the effects of environmental exposures as well as the role of epigenetics in human health. Illumina, Inc. recently replaced the HumanMethylation450 BeadChip (450K) with the EPIC BeadChip, which nearly doubles the measured CpG sites to 850,000. Although the new chip uses the same underlying technology, it is important to establish if data between the two platforms are comparable within cohorts and for meta-analyses. DNA methylation was assessed by 450K and EPIC using whole blood from newborn (n=109) and 14-year-old (n=86) participants of the Center for the Health Assessment of Mothers and Children of Salinas. The overall per-sample correlations were very high (r0.99), although many individual CpG sites, especially those with low variance of methylation, had lower correlations (median r=0.24). There was also a small subset of CpGs with large mean methylation -value differences between platforms, in both the newborn and 14-year datasets. However, estimates of cell type proportion prediction by 450K and EPIC were highly correlated at both ages. Finally, differentially methylated positions between boys and girls replicated very well by both platforms in newborns and older children. These findings are encouraging for application of combined data from EPIC and 450K platforms for birth cohorts and other population studies. These data in children corroborate recent comparisons of the two BeadChips in adults and in cancer cell lines. However, researchers should be cautious when characterizing individual CpG sites and consider independent methods for validation of significant hits.
机译：DNA甲基化的分析有助于了解环境暴露的影响以及表观遗传学对人体健康的作用。 Illumina，Inc。最近用史诗·珠芯片取代了人甲基化450珠芯片（450K），几乎将测量的CPG位点加倍，＆GT; 850,000。虽然新芯片使用相同的底层技术，但重要的是建立两个平台之间的数据是否在群组中和元分析中具有可比性。 DNA甲基化通过450K和史诗评估了来自新生儿（n = 109）和14岁（n = 86）的母亲和萨利纳斯儿童的健康评估中心的14岁（n = 86）参与者。整体每个样本相关性非常高（R> 0.99），尽管许多单独的CPG位点，尤其是甲基化方差差异低，相关性较低（中位r = 0.24）。在新生儿和14年的数据集中，还有一个小平均甲基化的CpGs的小型差异 - 平台之间的差异。然而，在两年龄段中，450K和史诗的细胞型比例预测的估计值高度相关。最后，男孩和女孩之间的差异甲基化的职位在新生儿和年龄较大的儿童中的平台上复制得很好。这些调查结果令人鼓舞的是在出生队列和其他人口研究中申请史诗和450K平台的组合数据。儿童中的这些数据证实了成人和癌细胞系中两珠芯片的最近比较。然而，在表征单个CPG网站时，研究人员应该是谨慎的，并考虑独立方法来验证大量击中。

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