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首页> 外文期刊>European journal of nutrition >Genetic relationships of boxwood (Buxus L.) accessions based on genic simple sequence repeat markers
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Genetic relationships of boxwood (Buxus L.) accessions based on genic simple sequence repeat markers

机译：基于遗传简易序列重复标记的菠菜（Buxus L.）涂布的遗传关系

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摘要

Boxwoods (Buxus L., Buxaceae) are popular woody landscape shrubs grown for their diverse forms and broad-leaved evergreen foliage. We used genic simple sequence repeat (genic-SSR) markers to assess genetic diversity and relatedness of 275 accessions from the National Boxwood Collection at the U.S. National Arboretum. Flow cytometry was conducted to determine the relative ploidy of each accession. Genic-SSR loci were highly variable among the accessions, detecting an average of 6.7 alleles per locus based on 17 primer pairs. Data were analyzed with a distance matrix based on Jaccard's similarity index, followed by Unweighted Pair Group Method with Arithmetic Mean clustering. Two major clusters were identified, with four subclusters consisting of individual accessions from B. balearica Lam., B. bodinieri L,vl., B. harlandii Hance, B. microphylla Siebold et Zuccarini, B. sempervirens L., B. sinica (Rehd. et Wils.) M. Cheng, and their putative interspecific hybrids. The accessions generally clustered by cultivar, provenance, or species. Clustering within each group typically reflected breeding pedigrees, when known, and the clusters were supported by bootstrap results. This information will be used for breeding programs and collection management, and for identifying possible sources of disease tolerance for boxwood blight and other diseases and pests.
机译：Boxwoods（Buxus L.，Bulaceae）是流行的木质景观灌木，为他们多样化的形式和阔叶常绿叶子种植。我们使用了基因简单序列重复（Genic-SSR）标记，以评估美国国家植物园国家植物园的全国菠菜馆收集遗传多样性和相关性。进行流式细胞术以确定每种加入的相对倍频性。 Genic-SSR基因座在附加过程中具有高度变量，基于17个引物对检测每个基因座的平均6.7个等位基因。基于Jaccard的相似性指数，用距离矩阵分析数据，然后是具有算术平均聚类的未加权对组方法。鉴定了两个主要集群，其中四个子平整板由B. Balearica Lam的单独载体组成，B.Bodinieri L，VL。，B. Harlandii Hance，B. Microphylla Siebold等Zuccarini，B. Sempervirens L.，B. Sinica（ REHD。et wils。）C. cheng，以及他们推定的三种杂种。饲养通常由品种，物种或物种聚集。在每个组内的聚类通常在已知的情况下反映繁殖百分比，并且通过引导结果支持群集。这些信息将用于培育方案和收集管理，并识别Bullwood枯萎病和其他疾病和害虫的可能疾病耐受源。

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