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首页> 外文期刊>European journal of clinical microbiology and infectious diseases: Official publication of the European Society of Clinical Microbiology >Evaluation of the Carba NP test for carbapenemase detection in Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp., and its practical use in the routine work of a national reference laboratory for susceptibility testing
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Evaluation of the Carba NP test for carbapenemase detection in Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp., and its practical use in the routine work of a national reference laboratory for susceptibility testing

机译：肠杆菌基乙基猕猴桃氨基氨基酶检测的CARBA NP试验评价。 和acinetobacter spp。及其在国家参考实验室的常规工作中的易感性测试的实际应用

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摘要

The aim of this study was to evaluate the Carba NP test (and CarbAcineto) for the detection of carbapenemases in Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp., and to assess its usefulness in the routine work of the National Reference Centre for Susceptibility Testing (NRCST) in Poland. The evaluation of the Carba NP/CarbAcineto tests was carried out on a group of 81 Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp. isolates producing KPC-, NDM-, VIM-, IMP- or OXA-48, -23, -24/40, -58-type carbapenemases, and on 26 carbapenemase-negative strains cultivated on a broad panel of microbiological media. Subsequently, the performance of the Carba NP/CarbAcineto tests was assessed on 1282 isolates of Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp. from Polish hospitals, submitted to the NRCST during a 9-month period in 2014. The Carba NP/CarbAcineto results were compared with other phenotypic tests and/or polymerase chain reaction (PCR). The impact of the media on the results of the Carba NP/CarbAcineto tests was observed, with the Columbia blood agar yielding the highest sensitivity and clarity of the results. Furthermore, the Carba NP/CarbAcineto tests were included in the NRCST routine procedure for carbapenemase identification. The sensitivity and specificity of the Carba NP test were 95.8% and 93.3%, respectively, for Enterobacteriaceae, and 97.5% and 99.0%, respectively, for Pseudomonas spp. The sensitivity of the CarbAcineto test for Acinetobacter spp. was 88.9%. This study confirmed the usefulness of the Carba NP/CarbAcineto tests for the rapid detection of various types of carbapenemases.
机译：本研究的目的是评估Carba NP试验（和Carbaceto），用于检测肠杆菌菌，假鼠SPP中的肠杆菌酶。和acinetobacter spp。，并评估波兰国家参考中心（NRCST）的日常工作中的实用性。对CARBA NP / CARBACINETO试验的评价在一组81个肠杆菌酵母，假单胞菌SPP中进行。和acinetobacter spp。分离物生产KPC-，NDM-，Vim - ，Imp-或Oxa-48，-23，-24 / 40，-58型碳结构酶，以及在宽的微生物培养基面板上栽培的26种Carbapenemase阴性菌株。随后，对1282分离株的肠杆菌，假霉菌，假霉素SPP评估了CARBA NP / CARBACINETO试验的性能。和acinetobacter spp。从波兰医院，在2014年的9个月内提交NRCST。与其他表型试验和/或聚合酶链反应（PCR）进行了比较Carba NP / Carbacineto结果。观察到培养基对Carba NP / Carbaceto试验结果的影响，哥伦比亚血琼脂产生最高的敏感性和结果清晰度。此外，CARBA NP / CARBACINETO试验包括在NRCST常规程序中用于碳碱酶酶鉴定。 Carba NP试验的敏感性和特异性分别为肠杆菌薄膜的95.8％和93.3％，分别为37.5％和99.0％，用于假单胞菌SPP。癌杆菌SPP的CARBACINETO试验的敏感性。是88.9％。该研究证实了Carba NP / Carbaceto试验的有用性，用于快速检测各种类型的碳结氨酸酶。

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