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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Frequency and immunophenotype of IL10-producing regulatory B cells in optic neuritis
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Frequency and immunophenotype of IL10-producing regulatory B cells in optic neuritis

机译：IL10产生的频率和免疫蛋白型在视神经炎中产生的调节B细胞

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Mouse models of multiple sclerosis (MS) have shown the importance of interleukin-10 (IL-10) -producing regulatory B (Breg) cells in dampening disease activity and inhibiting disease initiation and progression. In MS and other autoimmune diseases decreased frequency and functionality of Breg cells correlate with disease activity and the percentage of IL-10-producing Breg cells decreases during relapse and normalizes in remission. Optic neuritis (ON) is a common first clinical manifestation of MS and IL-10-producing Breg cells may be crucial in the transition from ON to MS, we therefore investigate the frequency and function of Breg cells in ON as a clinical model of early demyelinating disease. B cells were purified from 27 patients with ON sampled close to symptom onset (median 23 days, range 7-41 days) and 13 healthy controls. The B cells were stimulated and cultured for 48 hr with CD40 ligand and CpG before measurement of intracellular IL-10 and the surface markers CD19, CD1d, CD5, CD24, CD38 and CD27 by flow cytometry. The frequency of B-cell subsets was analysed in peripheral blood and cerebral spinal fluid (CSF) of patients. Sixty-five per cent of the IL-10-producing Breg cells co-expressed CD24 and CD38, and only 14% were CD24(high) CD27(+), suggesting that the naive B cells are the primary source of IL-10 in the B-cell culture, followed by memory cells in both healthy controls and patients. The frequency of naive CD19(+) CD24(+) CD38(+) Breg cells was higher in patients with ON compared with controls. The ability of Breg cells to produce IL-10 was at normal levels in both ON patients with high risk and those with low risk of progression to MS. We found no correlation between Breg cell function and the presence of brain white matter lesions by magnetic resonance imaging or CSF oligoclonal bands indicative of ON patients carrying a higher risk of conversion to MS. The frequencies of IL-10-producing B cells did not correlate with the conversion to MS at 2-year follow up. Interleukin-10 was primarily produced by naive and memory B cells. The frequency of IL-10-secreting B cells did not correlate with risk factors of MS. Breg cell function at clinical onset of ON is not a determining factor for conversion to MS.
机译：多发性硬化症（MS）的小鼠模型表明白细胞介素-10（IL-10） - 发挥调节B（BREG）细胞在阻尼疾病活动中的重要性和抑制疾病引发和进展的重要性。在MS和其他自身免疫疾病中，BREG细胞的频率和官能度与疾病活性相关，并且在缓解期间产生IL-10产生的BREG细胞的百分比降低。光学神经炎（ON）是MS和IL-10产生的临床表现的常见临床表现，其在从对MS的转变中可能是至关重要的，因此我们研究了BREG细胞作为早期临床模型的频率和功能脱髓鞘疾病。 B细胞从27例患者纯化，接近症状发作（中位数23天，7-41天）和13例健康对照。通过流式细胞术测量细胞内IL-10和表面标志物CD19，CD1D，CD5，CD24，CD38和CD27，刺激和培养B细胞和培养48小时的CD40配体和CPG。在患者的外周血和脑脊髓液（CSF）中分析了B细胞亚群的频率。六十五个产生的IL-10产生的Breg细胞CD24和CD38，仅14％是CD24（高）CD27（+），表明幼稚B细胞是IL-10的主要来源B细胞培养物，其次在健康对照和患者中的记忆细胞。与对照组相比，幼稚CD19（+）CD24（+）CD38（+）CD38（+）CD38（+）CD38（+）BREG细胞的频率较高。 BREG细胞生产IL-10的能力在高风险患者和患者对MS的患者身上的正常水平。我们发现BREG细胞功能与磁共振成像或CSF寡核条带的存在无相关性，指示患者转化为MS的患者的患者。 IL-10产生的B细胞的频率与2年后的转换与MS的转换不相关。白细胞介素-10主要由Naive和Memory B细胞产生。 IL-10分泌B细胞的频率与MS的危险因素不相关。临床发作的BREG细胞功能不是转换对MS的决定因素。

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