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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Obesity affects peripheral lymphoid organs immune response in murine asthma model
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Obesity affects peripheral lymphoid organs immune response in murine asthma model

机译：肥胖症影响小鼠哮喘模型中外周血淋巴器官免疫应答

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Summary Asthma and obesity present rising incidence, and their concomitance is a reason for concern, as obese individuals are usually resistant to conventional asthma treatments and have more exacerbation episodes. Obesity affects several features in the lungs during asthma onset, shifting the T helper type 2 ( Th 2)/eosinophilic response towards a Th 17/neutrophilic profile. Moreover, those individuals can present reduced atopy and delayed cytokine production. However, the impact of obesity on follicular helper T ( Tfh ) cells and B cells that could potentially result in antibody production disturbances are still unclear. Therefore, we aimed to assess the peripheral response to ovalbumin ( OVA ) in a concomitant model of obesity and asthma. Pulmonary allergy was induced, in both lean and obese female BALB/c mice, through OVA sensitizations and challenges. Mediastinal lymph nodes ( MLN s) and spleen were processed for immunophenotyping. Lung was used for standard allergy analysis. Obese‐allergic mice produced less anti‐ OVA IgE and more IgG2a than lean‐allergic mice. Dendritic cells ( CD 11c + ? MHCII high ) expressed less CD 86 and more PDL 1 in obese‐allergic mice compared with lean‐allergic mice, in the MLN s. Meanwhile, B cells ( CD 19 + ? CD 40 + ) were more frequent and the amount of PDL 1/ PD 1 + cells was diminished by obesity, with the opposite effects in the spleen. Tfh cells ( CD 3 + ? CD 4 + ? CXCR 5 + ? PD 1 + ) expressing FoxP3 were more frequent in obese mice, associated with the predominance of Th ( CD 3 + ? CD 4 + ) cells expressing interleukin‐4/ GATA 3 in the MLN s and interleukin‐17A/ ROR γ T in the spleen. Those modifications to the main components of the germinal centers could be resulting in the increased IgG2a production, which?–?associated with the Th 17/neutrophilic profile?–?contributes to asthma worsening and represents an important target for future treatment strategies.
机译：总结哮喘和肥胖目前出现的发病率升高，而他们的同意是令人担忧的原因，因为肥胖个体通常耐常见的哮喘治疗，并且具有更加恶化的发作。肥胖症在哮喘发作期间影响肺部的几个特征，将T辅助型2（TH 2）/嗜酸性渗透反应移位为TH 17 /嗜中性曲线。此外，这些个体可以提高降低的特性和延迟细胞因子产生。然而，肥胖症对卵泡辅助T（TFH）细胞和B细胞的影响仍不清楚。因此，我们的目标是在伴随肥胖和哮喘的伴随模型中评估对卵磷酸酯（OVA）的外周反应。肺部过敏诱导瘦弱和肥胖女性Balb / C小鼠，通过OVA敏感和挑战。处理纵隔淋巴结（MLN S）和脾脏用于免疫蛋白酶型。肺被用于标准过敏分析。肥胖 - 过敏小鼠比贫过敏小鼠产生较少的抗OVA IgE和更多IgG2a。在MLN S中，与贫肺过敏小鼠相比，树突状细胞（CD 11C +ΔMHCII高）在肥胖 - 过敏小鼠中表达了较少的CD 86和更多PDL 1。同时，B细胞（CD19 +ΔCD40 +）更频繁，PDL 1 / Pd1 +细胞的量通过肥胖降低，脾脏效果相反。表达Foxp3的TFH细胞（CD 3 +ΔCD4 +ΔCXCR5 +βPD1+）在肥胖小鼠中更频繁地频繁，与表达白细胞介素-4 / GATA的TH（CD 3 +ΔCD4 +）细胞的主要相关3在脾中的MLN S和白细胞介素-17a /RORγT中。这些对生发中心的主要成分的修饰可能导致IgG2A的产生增加，这是α - - ？与Th 17 /中性粒细胞曲线相关的影响？ - ？有助于哮喘恶化，代表未来治疗策略的重要目标。

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