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首页> 外文期刊>European Journal of Plant Pathology >Response of PIWI grapevine cultivars to downy mildew in highland region of southern Brazil
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Response of PIWI grapevine cultivars to downy mildew in highland region of southern Brazil

机译:巴西南部高地地区Piwi葡萄葡萄种栽对霜霉病的反应

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摘要

Downy mildew (DM) caused by Plasmopara viticola is a major grapevine disease in southern Brazil. Control of DM requires frequent fungicide treatments or the use of resistant cultivars. The pyramiding of previously identified resistance loci allows the selection of genotypes with combined resistance loci in order to build up sustainable resistance. The objective of this study was to evaluate the resistance response of PIWI cultivars Felicia, Bronner, and Calardis Blanc, containing Rpv3.1, Rpv10, and Rpv3.1 + Rpv3.2 loci, respectively, and two advanced breeding selections (ABS) containing the Rpv1 + Rpv3.1 pyramided, to the development of DM temporal dynamics. The experiment was conducted at an experimental vineyard in the highland of southern Brazil, during the 2016/2017 and 2017/2018 growing seasons. The genotype UFSC-2012-1-24 without resistance loci was used as susceptible control. DM incidence and severity were assessed biweekly from the first symptoms appearance until harvest. The cultivars and ABS were compared based on the following DM epidemiological parameters: a) beginning of symptoms appearance (BSA); b) maximum disease incidence and severity (I-max, S-max); c) time to reach maximum disease incidence/severity (TRMDI and TRMDS); and d) areas under incidence and severity disease progress curves (AUIDPC and AUSDPC). In general, there were significant differences in the estimated parameters depending on the genotype. The principal epidemiological variables that differentiated the PIWI cultivars were S-max and AUSDPC. The PIWI cultivars showed significantly lower S-max and AUSDPC of DM in comparison to the susceptible control in both 2016/2017 and 2017/2018 growing seasons. There were also significant differences between the cultivars for AUIDPC and AUSDPC in both growing seasons. Felicia cultivar showed the highest AUIDPC and AUSDPC in the 2016/2017 growing season. Surprisingly, in the growing seasons 2017/2018, Calardis Blanc displayed an increase in disease incidence and severity, showing a similar AUIDPC and AUSDPC to Felicia cultivar. In general, Bronner cultivar was the most resistant to DM. The ABS showed the lowest value of I-max, S-max, AUIDPC and AUSDPC, suggesting the effectiveness of the Rpv1 + Rpv3.1 pyramiding. The PIWI cultivars showed partial resistance to DM and are potential options for reducing fungicide use. The results showed that the pyramiding of resistance loci is fundamental to increase the genetic resistance to DM.
机译:由Plasmopara Viticola引起的霜霉病(DM)是巴西南部的主要葡萄病。 DM的控制需要频繁的杀菌剂处理或使用抗性品种。先前识别的抗性基因座的功率允许选择具有组合电阻基因座的基因型,以便增加可持续的抗性。本研究的目的是评估PIWI品种Felicia,Bronner和Calardis Blanc,含有RPV3.1,RPV10和RPV3.1 + RPV3.2基因座的抗性响应,以及含有两种先进的育种选择(ABS) RPV1 + RPV3.1赞聚,以发展DM时间动态。在2016/2017年和2017/2018生长季节,在巴西南部的高地的实验葡萄园进行了实验。没有抗性基因座的基因型UFSC-2012-1-24用作敏感控制。 DM发病率和严重程度从第一个症状出现之前评估,直至收获。基于以下DM流行病学参数进行比较品种和ABS:a)症状出现的开始(BSA); b)最大疾病发病率和严重程度(I-Max,S-Max); c)达到最大疾病发生率/严重程度的时间(TRMDI和TRMD);和d)发病率和严重疾病进展曲线(Auidpc和ausdpc)的区域。通常,根据基因型,估计参数存在显着差异。将PIWI品种分化的主要流行病学变量是S-MAX和AUSDPC。与2016/2017和2017/2018生长季节的易感控制相比,PIWI栽培品种显着降低了DM的S-MAX和AUSDPC。在繁殖季节的Auidpc和Ausdpc品种之间也存在显着差异。 Felicia品种在2016/2017生长季节显示了最高的Auidpc和Ausdpc。令人惊讶的是,在2017/2018的生长季节,Calardis Blanc展现出疾病发病率和严重程度的增加,显示出类似的Auidpc和Ausdpc到Felicia品种。一般来说,布朗纳品种对DM最有抗性。 ABS显示I-MAX,S-MAX,AUIDPC和AUSDPC的最低值,表明RPV1 + RPV3.1功率的有效性。 PIWI品种表现出对DM的部分抗性,并且是减少杀菌剂使用的潜在选择。结果表明,抗性基因座的功率是增加对DM的遗传性抗性的基础。

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