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首页> 外文期刊>European Journal of Plant Pathology >Development and characterization of EST-derived SSR markers in the cereal cyst nematode Heterodera avenae
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Development and characterization of EST-derived SSR markers in the cereal cyst nematode Heterodera avenae

机译：谷物囊肿yematode yevenae中EST衍生SSR标记的开发与表征

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摘要

The cereal cyst nematode Heterodera avenae is a pathogen of cereal crops and causes high yield losses worldwide. In this study, a collection of 37,348 expressed sequence tags (ESTs) of H. avenae was mined for EST-based simple sequence repeat (SSR) markers, which resulted in the identification of 5604 SSRs. A total of 210 pairs of SSR primers were further developed and used for validation of the amplification rate and assessment of the polymorphism. Eight SSR markers were finally identified and analyzed using 96 individual cysts of H. avenae sampled from three provinces in China. These loci were found to be moderately polymorphic with 3-8 alleles per locus. The observed and expected heterozygosity across the three populations ranged from 0.000 to 0.594 and from 0.000 to 0.731, respectively. The polymorphism information content (PIC) was medium and ranged from 0.080 to 0.562, with a mean of 0.409. The F (ST) ranged from 0.1034 to 0.1550, indicating moderate genetic differentiation among the three H. avenae populations. These EST-SSR markers can be used to study population genetic diversity and the dispersal route of H. avenae in China.
机译：谷物Cyst Nematode heterodera Avenae是谷物作物的病原体，并在全球范围内引起高产损失。在该研究中，为EST的简单序列重复（SSR）标记进行H.Avenae的37,348种表达序列标签（EST）的集合，导致鉴定为5604SSR。还进一步开发了总共210对SSR引物，用于验证对多态性的扩增速率和评估。最后鉴定了八个SSR标记，并使用来自中国三个省份的H. Avenae的96个个体囊肿分析。发现这些基因座是适度的多态性，每个基因座为3-8个等位基因。观察到的和预期的杂合性分别为0.000〜0.594和0.000至0.731。多态性信息含量（PIC）是培养基，范围为0.080至0.562，平均为0.409。 F（ST）的范围为0.1034至0.1550，表明三个H. Avenae种群中的中度遗传分化。这些EST-SSR标记可用于研究群体遗传多样性和中国H. Avenae的分散路径。

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    Wang X.; Ma J.; Liu H.; Liu R.; Li H.;

  • 作者单位

    Nanjing Agr Univ Dept Plant Pathol Key Lab Integrated Management Crop Dis &

    Pestse Minist Educ Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Dept Plant Pathol Key Lab Integrated Management Crop Dis &

    Pestse Minist Educ Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Dept Plant Pathol Key Lab Integrated Management Crop Dis &

    Pestse Minist Educ Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Dept Plant Pathol Key Lab Integrated Management Crop Dis &

    Pestse Minist Educ Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Dept Plant Pathol Key Lab Integrated Management Crop Dis &

    Pestse Minist Educ Nanjing 210095 Jiangsu Peoples R China;

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  • 正文语种 eng
  • 中图分类 植物病理学;
  • 关键词

    Microsatellite markers; Phytoparasitic nematode; Polymorphism analysis; Population genetics;

    机译：微卫星标志物;植物肝炎线虫;多态性分析;人口遗传学;

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