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首页> 外文期刊>Experimental and therapeutic medicine >C/EBP homologous protein (CHOP) mediates neuronal apoptosis in rats with spinal cord injury
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C/EBP homologous protein (CHOP) mediates neuronal apoptosis in rats with spinal cord injury

机译：C / EBP同源蛋白（Chec）在脊髓损伤大鼠中介导神经元细胞凋亡

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摘要

Spinal cord injury (SCI) is a severe health problem and the mechanism involved remains elusive. The aim of the present study was to elucidate the role of C/EBP homologous protein (CHOP), a prominent protein of the endoplasmic reticulum (ER) stress-mediated apoptosis in SCI. A total of 20 adult male Sprague-Dawley rats were divided into two groups at random, ten rats were subjected to a modified Allen's test (using a weight-drop device) to induce a SCI model and the remaining ten rats only had the corresponding vertebral lamina removed with no injury and served as the sham-operated group. Pathological changes in the spinal cord were observed 12 h after injury by hematoxylin and eosin staining and TUNEL staining was performed to visualize apoptotic cells. The expression of CHOP was also detected by immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction. The results showed that a typical apoptotic morphology, namely the increased the number of TUNEL-positive cells in the injured spinal cord. The expression levels of CHOP in the rats with SCI were increased compared with the sham-operated rats (P<0.05). These results revealed that CHOP-mediated ER stress-induced apoptosis may be involved in SCI.
机译：脊髓损伤（SCI）是一个严重的健康问题，涉及的机制仍然难以捉摸。本研究的目的是阐明C / EBP同源蛋白（CHOP），内质网（ER）胁迫介导的SCI中的突出蛋白质的作用。总共20名成年雄性Sprague-Dawley大鼠随机分为两组，将十只大鼠进行修饰的艾伦的测试（使用重量滴剂）诱导SCI模型，其余十只大鼠仅具有相应的椎骨Lamina除去没有伤害并用作假手术组。通过苏木精损伤和曙红染色和TUNEL染色以观察到肌蛋白染色和TUNEL染色以显示凋亡细胞后，观察到脊髓的病理变化。还通过免疫组织化学和定量实时逆转录 - 聚合酶链反应检测剁的表达。结果表明，典型的凋亡形态，即增加了受伤脊髓中的动脉阳性细胞的数量。与假手术大鼠相比，对SCI大鼠的斩块的表达水平增加（P <0.05）。这些结果表明，切碎介导的ER应激诱导的细胞凋亡可参与SCI。

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