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首页> 外文期刊>Experimental Neurology >Ulinastatin protects brain against cerebral ischemia/reperfusion injury through inhibiting MMP-9 and alleviating loss of ZO-1 and occludin proteins in mice
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Ulinastatin protects brain against cerebral ischemia/reperfusion injury through inhibiting MMP-9 and alleviating loss of ZO-1 and occludin proteins in mice

机译：Ulinastatin通过抑制MMP-9和小鼠中的ZO-1和occludin蛋白质的损失来保护脑脑缺血/再灌注损伤免受脑缺血/再灌注损伤

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摘要

Background: The effects of Ulinastatin (UTI) on the blood-brain barrier (BBB) in the acute phase of cerebral ischemia/reperfusion (I/R) are not clear. This study was to investigate the potential protective effects of UTI on the BBB and the underlying mechanisms.& para;Methods: Male CD-1 mice were subjected to transient middle cerebral artery occlusion (tMCAO) and randomly assigned to four groups: Sham (sham-operated), tMCAO (tMCAO + 0.9% saline), UTI-L (tMCAO + UTI 1500 U/100 g) and UTI-H (tMCAO + UTI 3000 U/100 g) group. UTI was administered immediately after reperfusion in the UTI-L and UTI-H groups. At 24 h after reperfusion, the neurological deficit, brain water content, and infarct volume were determined. Western blot and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were used to examine the expression of matrix metalloproteinase (MMP)-9, Zonula occludens-1 (ZO-1) and occludin in ischemic cerebral cortex. The integrity of the BBB was assessed by the leakage of Evans blue. & para;& para;Results: Compared with tMCAO group, both UTI-L and UTI-H groups showed significantly (P 0.001) ameliorated the neurological deficit (2.00 +/- 0.71 and 1.60 +/- 0.55 vs. 4.60 +/- 0.55), lessened brain water content (82.99% +/- 0.21% and 82.05% +/- 0.59% vs. 84.28% +/- 0.0.57%) and decreased the infarct volume (38.52% +/- 1.72% and 24.78% +/- 1.20% vs. 49.48% +/- 1.93%). In addition, significantly (P 0.001) decreased expression of MMP-9 (0.48 0.06 and 0.37 +/- 0.05 vs.0.76 +/- 0.10 for protein and 2.88 +/- 0.23 and 2.17 +/- 0.16 vs. 3.90 +/- 0.24 for mRNA) and alleviated loss of ZO-1 (0.19 +/- 0.04 and 0.24 0.05 vs. 0.25 +/- 0.03) and occludin (0.74 +/- 0.08 and 0.87 +/- 0.07 vs. 0.94 +/- 0.06) proteins were observed in both UTI-L and UTI-H groups.& para;& para;Conclusion: UTI protects the brain against ischemic injury potentially via down-regulating the expression of MMP-9 and alleviating loss of ZO-1 and occludin proteins to restore the BBB permeability.
机译：背景：乌司汀（UTI）对脑缺血/再灌注（I / R）急性期血脑屏障（BBB）的影响尚不清楚。本研究是探讨UTI对BBB和潜在机制的潜在保护作用。＆段;方法：对阳性CD-1小鼠进行瞬时中间脑动脉闭塞（TMCAO），随机分配到四组：假（假-operated），TMCAO（TMCAO + 0.9％盐水），UTI-L（TMCAO + UTI 1500 U / 100g）和UTI-H（TMCAO + UTI 3000 U / 100g）组。在再灌注到UTI-L和UTI-H组后立即施用UTI。在再灌注后24小时，确定神经缺陷，脑含水量和梗塞体积。用于检测缺血性脑皮层中基质金属蛋白酶（MMP）-9，ZO-1）和闭塞素的基质金属蛋白酶（MMP）-9，ZO-1）和闭塞蛋白的表达的表达。通过埃文斯蓝的泄漏来评估BBB的完整性。结果：结果：与TMCAO组相比，UTI-L和UTI-H组都显着显示出来（P <0.001）改善神经缺陷（2.00 +/- 0.71和1.60 +/- 0.55与4.60 + / - 0.55），减少脑含水量（82.99％+/- 0.21％和82.05％+/- 0.59％与84.28％+/- 0.0.57％）并降低梗塞体积（38.52％+/- 1.72％和24.78％+/- 1.20％与49.48％+/- 1.93％）。此外，显着（P <0.001）显着降低MMP-9的表达（0.48.06和0.37 +/- 0.05 Vs.0.76 +/- 0.10，2.88 +/- 0.23和2.17 +/- 0.16与3.90 + / - mRNA的0.24），缓解ZO-1（0.19 +/- 0.04和0.24 0.05 vs.0.25 +/- 0.03）和occludin（0.74 +/- 0.08和0.87 +/- 0.07和0.94 +/-在UTI-L和UTI-H组中观察到蛋白质。＆para;＆para;结论：UTI潜在地通过降低MMP-9的表达来保护大脑免受缺血性损伤，并减轻ZO-1的丧失Occludin蛋白恢复BBB渗透性。

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  • 来源
    《Experimental Neurology》 |2018年第2018期|共7页
  • 作者

    Li Xiao-Fang; Zhang Xiang-Jian; Zhang Cong; Wang Li-Na; Li Yao-Ru; Zhang Ye; He Ting-Ting; Zhu Xing-Yuan; Cui Li-Li; Gao Bu-Lang;

  • 作者单位

    Hebei Med Univ Hosp 2 Dept Neurol 215 West Heping Rd Shijiazhuang 050000 Hebei Peoples R China;

    Hebei Med Univ Hosp 2 Dept Neurol 215 West Heping Rd Shijiazhuang 050000 Hebei Peoples R China;

    Hebei Med Univ Hosp 2 Dept Neurol 215 West Heping Rd Shijiazhuang 050000 Hebei Peoples R China;

    Hebei Med Univ Hosp 2 Dept Neurol 215 West Heping Rd Shijiazhuang 050000 Hebei Peoples R China;

    Hebei Key Lab Vasc Homeostasis Shijiazhuang Hebei Peoples R China;

    Hebei Key Lab Vasc Homeostasis Shijiazhuang Hebei Peoples R China;

    Hebei Key Lab Vasc Homeostasis Shijiazhuang Hebei Peoples R China;

    Hebei Key Lab Vasc Homeostasis Shijiazhuang Hebei Peoples R China;

    Hebei Med Univ Hosp 2 Dept Neurol 215 West Heping Rd Shijiazhuang 050000 Hebei Peoples R China;

    Henan Prov Peoples Hosp Dept Intervent Treatment Zhengzhou Henan Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 神经病学;
  • 关键词

    Cerebral ischemia/reperfusion; Ulinastatin; Matrix metalloproteinase-9; Zonula occludens-1; Occludin;

    机译：脑缺血/再灌注;乌氏菌素;基质金属蛋白酶-9;Zonula Occludens-1;occludin;

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