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首页> 外文期刊>Experimental Eye Research >Recovery of the sub-basal nerve plexus and superficial nerve terminals after corneal epithelial injury in mice
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Recovery of the sub-basal nerve plexus and superficial nerve terminals after corneal epithelial injury in mice

机译：在小鼠角膜上皮损伤后的亚基神经丛和浅表神经末端的恢复

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Our aim was to compare regeneration of the sub-basal nerve plexus (SBNP) and superficial nerve terminals (SNT) following corneal epithelial injury. We also sought to compare agreement when quantifying nerve parameters using different image analysis techniques. Anesthetized, female C57BL/6 mice received central 1-mm corneal epithelial abrasions. Four-weeks post-injury, eyes were enucleated and processed for PGP9.5 to visualize the corneal nerves using wholemount immunofluorescence staining and confocal microscopy. The percentage area of the SBNP and SNT were quantified using: ImageJ automated thresholds, ImageJ manual thresholds and manual tracings in NeuronJ. Nerve sum length was quantified using NeuronJ and Imaris. Agreement between methods was considered with Bland-Altman analyses. Four-weeks post-injury, the sum length of nerve fibers in the SBNP, but not the SNT, was reduced compared with naive eyes. In the periphery, but not central cornea, of both naive and injured eyes, nerve fiber lengths in the SBNP and SNT were strongly correlated. For quantifying SBNP nerve axon area, all image analysis methods were highly correlated. In the SNT, there was poor correlation between manual methods and auto-thresholding, with a trend towards underestimating nerve fiber area using auto-thresholding when higher proportions of nerve fibers were present. In conclusion, four weeks after superficial corneal injury, there is differential recovery of epithelial nerve axons; SBNP sum length is reduced, however the sum length of SNTs is similar to naive eyes. Care should be taken when selecting image analysis methods to compare nerve parameters in different depths of the corneal epithelium due to differences in background autofluorescence.
机译：我们的目的是在角膜上皮损伤之后比较亚基神经丛（SBNP）和浅表神经末端（SNT）的再生。我们还试图使用不同的图像分析技术量化神经参数时比较协议。麻醉的雌性C57BL / 6小鼠接受了中央1-MM角膜上皮擦伤。损伤后四周，眼睛对PGP9.5加工并加工，使用全新荧光染色和共聚焦显微镜来可视化角膜神经。使用：imagej自动阈值，imagej自动阈值和neuronj中的手动曲线来量化SBNP和SNT的百分比面积。使用Neuronj和Imaris量化神经和长度。与Bland-Altman分析考虑了方法之间的协议。损伤后四周，SBNP中神经纤维的总长度，但不是SNT，与幼稚的眼睛相比减少。在幼稚和受伤的眼睛的外围，但不是中央角膜中，SBNP和SNT中的神经纤维长度都强烈相关。对于量化SBNP神经轴突区域，所有图像分析方法都具有高度相关性。在SNT中，手动方法与自动阈值之间的相关性差，当存在较高比例的神经纤维时，在存在自动阈值时，具有趋势趋势。总之，浅表角膜损伤四周，上皮神经轴突有差异恢复; SBNP SUM长度减少，但是SNT的总长度与幼稚的眼睛类似。在选择图像分析方法时应注意，以比较角膜上皮细胞不同深度的神经参数，由于背景自发荧光的差异。

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