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首页> 外文期刊>Experimental parasitology >Cell viability analysis of Toxocara cati larvae with LIVE / DEAD (R) Viability/Cytotoxicity kit
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Cell viability analysis of Toxocara cati larvae with LIVE / DEAD (R) Viability/Cytotoxicity kit

机译：用活/死（R）活力/细胞毒性套件的毒素卡西幼虫的细胞活力分析

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Toxocara spp. are responsible for causing toxocariasis, a zoonotic disease of global significance. In some countries of South America, toxocariasis is considered the most prevalent human helminthic infection. The objective of this study was to evaluate LIVE/DEAD (R) Viability/Cytotoxicity kit as an alternative method to analyze the viability of Toxacara cad larvae. Two control groups were used to confirm the usage of this methodology: 100 untreated T. cad larvae as a negative control (G1) and 100 T. cad larvae killed by thermal shock as a positive control (G2). Subsequently, the viability of T. cad larvae was assessed by the exclusion of the trypan blue dye and by LIVE/DEAD (R) Viability/Cytotoxicity kit, as well as observation of motility and morphology. In order to confirm the larvicidal effect, T. cad larvae G1 and G2 were inoculated in mice to evaluate their progression in vivo. As expected, G1 showed negative staining by Trypan blue and was stained green by LIVE/DEAD (R) Viability/Cytotoxicity kit in all the exposure periods. Moreover, G1 presented 100% of relative motility (RM) (score of 5). G2 group was stained blue by Trypan blue and red by LIVE/DEAD (R) Viability/Cytotoxicity kit, and had 0% RM (score zero) in 24 h of incubation period. In mice, G2 was not viable and, therefore, was not able to infect the animals. In mice inoculated with G1, however, larvae were recovered from all the evaluated organs, except eyes. These results demonstrate that the viability of T. cad larvae was accurately obtained by the LIVE/DEAD (R) Viability/Cytotoxicity kit, making it an alternative method for viability evaluation.
机译：托克索卡SPP。负责导致毒性病毒，一种全球意义的一种人群疾病。在南美洲的一些国家，毒性病患者被认为是最普遍的人类蠕虫感染。本研究的目的是评估Live / Dead（R）活力/细胞毒性试剂盒作为分析毒素CAD幼虫的活力的替代方法。使用两组对照组来证实该方法的使用：100个未经处理的T. CAD幼虫作为阴性对照（G1）和100吨CAD幼虫作为阳性对照（G2）杀死。随后，通过排除Trypan Blue Dye和Live / Dead（R）活力/细胞毒性试剂盒来评估T. CAD幼虫的活力，以及观察运动和形态。为了确认幼虫效应，将CAD幼虫G1和G2接种在小鼠中以评估其体内进展。正如预期的那样，G1通过Trypan Blue显示阴性染色，并且在所有接触期内通过活/死（R）活力/细胞毒性套件染色绿色。此外，G1呈现100％的相对运动（RM）（得分为5）。 G2组通过锥虫蓝色和红色通过Live / Dead（R）活力/细胞毒性套件染色，并且在孵化期的24小时内具有0％RM（得分为零）。在小鼠中，G2不可行，因此，不能感染动物。然而，在接种G1的小鼠中，除了眼睛外，从所有评估的器官中回收幼虫。这些结果表明，通过活/死（R）活力/细胞毒性试剂盒精确地获得CAD幼虫的可行性，使其成为可行性评估的替代方法。

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