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首页> 外文期刊>Genetic Resources and Crop Evolution >Germplasm collection, genetic diversity and on-farm conservation of cowpea [Vigna unguiculata (L.) Walp.] landraces from Apulia region (southern Italy)
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Germplasm collection, genetic diversity and on-farm conservation of cowpea [Vigna unguiculata (L.) Walp.] landraces from Apulia region (southern Italy)

机译：豇豆种质收集，遗传多样性和农场保护[Vigna Unguiculata（L.）沃尔普。

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摘要

Cowpea [Vigna unguiculata (L.) Walp.] is a major tropical pulse in a number of countries and especially in sub-Saharan Africa where this species was domesticated. Although as niche product, a remarkable number of cowpea landraces are cultivated in the Mediterranean basin, primarily for human consumption of the grains and fresh pods. In this study, within the frame of a regional project devoted to the safeguard and management of local horticultural genetic resources, some germplasm explorations were carried out in Apulia region (southern Italy) to collect, among other crops, cowpea landraces still present in the area and promote on-farm conservation of this traditional material. A second objective was to investigate the efficiency of SSR markers in detecting genetic polymorphism in cowpea landraces from a restricted geographical area. In total, 13 samples of V. unguiculata were gathered, 12 of cultivar group unguiculata and 1 of cultivar group sesquipedalis. Genetic diversity and phylogenetic relationships among 13 Apulian accessions, 9 samples from various provenances and 1 var. spontanea accession were evaluated using SSR molecular markers. A set of 19 SSR primer pairs successfully identified closely related accessions and were able to separate the different cultivar groups of V. unguiculata.
机译：豇豆[Vigna Unguiculata（L.）Walp。]是许多国家的主要热带脉搏，特别是在撒哈拉以南非洲，其中该物种驯化。虽然作为利基产品，但在地中海盆地中培养了巨大的豇豆样力，主要用于谷物和新鲜豆荚的人类食用。在本研究中，在一个致力于保护和管理当地园艺遗传资源的区域项目的框架内，一些种质探索是在普利亚地区（意大利南部）进行的，尤其是作物，豇豆地铁仍然存在于该地区并促进这种传统材料的农场保护。第二个目的是探讨SSR标志物在受限制地理区域检测豇豆地球群中遗传多态性的效率。总共收集了13种V. unguiculata样品，12种苗族群和1种品种群Sesquipedalis。遗传多样性和系统发育关系中的13例普利亚患者，来自各种常产的9个样品和1次。使用SSR分子标记评估Spontanea附加型。一组19个SSR引物对成功确定了密切相关的载体，并且能够将不同的品种群体与unguiculata分开。

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