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首页> 外文期刊>Genetic Resources and Crop Evolution >Patterns of mitochondrial DNA fragmentation in bread wheat (Triticum aestivum L.) seeds under ex situ genebank storage and artificial aging
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Patterns of mitochondrial DNA fragmentation in bread wheat (Triticum aestivum L.) seeds under ex situ genebank storage and artificial aging

机译：面包小麦（Triticum aestivum L.）种子的线粒体DNA碎片碎片的模式在出原位基因库储存和人工老龄化下

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摘要

Monitoring viability loss of seeds conserved ex situ for timely germplasm regeneration is a challenging task in germplasm management and conservation as the process of seed deterioration under long-term storage remains poorly understood and effective tools for assessing seed deterioration are lacking. This paper reports findings from four experiments that were aimed to study the fragmentation of mitochondrial DNA (mtDNA) due to degradation in naturally or artificially aged (NA or AA) wheat seeds. Together, it was found that mtDNA fragmentation occurred in aged wheat seeds. More mtDNA fragmentation was observed in AA than NA seeds and degradation was more prominent in abnormal seedling tissues than in normal seedling tissues. Fragmentation of mtDNA was linearly associated with sample germination; more in less viable seed samples. Mitochondria (mt) in aged seeds were found to break throughout the mitochondrial genome with the observed loss of PCR amplification of atp1-5, nad4.2, atp4, and nad9 gene loci and at the roughly 80 kb repetitive region. These findings are useful for understanding the process and involvement of mtDNA degradation in NA or AA wheat seeds and for further exploration to develop aging biomarkers for monitoring seed viability.
机译：监测种子的活力丧失保守原地用于及时种质再生是一种挑战性的任务，在种质管理和节约中，随着长期储存的种子恶化的过程仍然缺乏，缺乏用于评估种子恶化的有效工具。本文报告了四种实验的结果，该试验旨在研究线粒体DNA（MTDNA）的碎片由于在天然或人工老化（NA或AA）小麦种子中的降解。共同发现，在老年的小麦种子中发生MTDNA碎片。在AA中观察到更多MTDNA碎片，而不是Na种子，并且在幼苗组织异常突出的降解比在正常的幼苗组织中更突出。 MTDNA的碎片与样品萌发线性相关;更多在不那么可行的种子样本中。发现在衰老的种子中的线粒体（MT）在整个线粒体基因组中，观察到的ATP1-5，NAD4.2，ATP4和NAD9基因座的PCR扩增丧失以及大约80kb的重复区域。这些发现可用于理解MTDNA降解在NA或AA小麦种子中的过程和涉及和进一步探索，以开发用于监测种子活力的老化生物标志物。

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