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首页> 外文期刊>Genetic Resources and Crop Evolution >Cross-transferability analysis of SSR markers developed from the American Cranberry (Vaccinium macrocarpon Ait.) to other Vaccinium species of agricultural importance
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Cross-transferability analysis of SSR markers developed from the American Cranberry (Vaccinium macrocarpon Ait.) to other Vaccinium species of agricultural importance

机译：从美国蔓越莓（Vaccinium Macrocarpon Ait，SSR标记的交叉转移性分析

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摘要

Cranberry (Vaccinium macrocarpon Ait.) breeding is evolving as consumer demand increases for specialty products such as sweetened and dried cranberries. New varieties are needed with not only higher yield and better adaptations to biotic (e.g., fungal fruit rot) and abiotic (e.g., cold tolerance) stressors, but also with increased fruit quality for processing and human nutrition. Cranberry is one of the few species in the genus Vaccinium that is utilized as a crop. However, the genus is composed of many understudied and underused species, such as wild blueberries and cranberries, lingonberries, and deerberries. Many of these species are cross-compatible and possess an array of traits of high agronomical value that may be commercially exploited through intrasectional or intersectional interspecific breeding. In order to provide a toolset for Vaccinium breeders, we tested the cross-transferability of 507 V. macrocarpon simple sequence repeat (SSR) markers on 17 different Vaccinium species. We found 61 SSR markers that consistently amplified and produced scorable bands across all 17 species tested. The ability of the markers to discriminate species based on their genetic relationships was tested for future use in phylogenetics. We genetically discriminated the different species and sections of the genus, providing an insightful look into the genetic relationship of species in this genus. These markers represent a working set of SSRs to use for the development of Vaccinium interspecific hybrids and to allow the development of population genetic studies of poorly understood species.
机译：蔓越莓（vaccinium macrocarpon ait。）育种正在发展，因为消费者需求增加了甜味和干蔓越莓等特种产品。不仅需要更高的产量和更好地适应生物（例如，真菌果实腐烂）和非生物（例如，冷耐腐蚀）压力源，而且需要增加新品种，而且还随着加工和人类营养的水果质量增加。蔓越莓是属性疫苗中的少数物种之一，用于作为作物使用。然而，该属由许多被覆盖物和未充分利用的物种组成，例如野生蓝莓和蔓越莓，鳞茎和陶伯里斯。这些物种中的许多是交叉兼容的并且具有通过含有含有型号或交叉间隙育种的高等农业价值的特征阵列。为了提供用于疫苗育种者的工具集，我们在17种不同的疫苗物种上测试了507 V.Mmrocarpon简单序列重复（SSR）标记的交叉转移性。我们发现了61个SSR标记，可在所有测试的所有17种物种中始终如一地放大和生产的可批量带。测试标记基于其遗传关系的鉴别物种的能力被测试用于将来在系统发育中使用。我们遗传地歧视了属的不同物种和部分，提供了富有洞察力的思想在该属中物种的遗传关系。这些标志物代表了用于开发疫苗三种杂种的SSR的工作组，并允许遗传遗传研究的发展不良物种。

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