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首页> 外文期刊>Genetic Resources and Crop Evolution >Diversity and distribution of Rc1 alleles in Triticum urartu from the Middle East
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Diversity and distribution of Rc1 alleles in Triticum urartu from the Middle East

机译：从中东地区rc1等位基因RC1等位基因的多样性和分布

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摘要

Rc1 (red coleoptile) encode a R2R3-MYB transcription factor TaMYB-A1 in common wheat, but the homologous genes have not been reported in Triticum urartu or Triticum monococcum, both species being sources of the ancient A genome. In this study, four alleles were discovered in 120 T. urartu accessions, and two alleles from nine T. monococcum accessions. The six alleles encoded five proteins because TuMYB-A1-F and TuMYB-A1-C encoded the same protein. All mutated sites in the amino acid sequence of these alleles were out of the R2R3 and transcript activator domains, and did not affect the conserved tryptophan (W) residues nor the "D/ELX2R/KX3LX6LX3R" motif of the MYB proteins. The alleles from T. monococcum were more similar in sequence to TaMYB-B1 and TaMYB-D1 than were those from T. urartu in the phylogenetic tree, while the alleles from T. urartu resembled TaMYB-A1 more closely than did the T. monococcum alleles. TuMYB-A1-C was the most widely distributed allele, and Turkey contained the greatest genetic variation compared with the five other countries in the geographical distribution analysis. Transient expression of TuMYB-A1-D or TuMYB-A1-F induced anthocyanin biosynthesis in white coleoptiles of wheat cultivar 'Opata' with the help of ZmR. RT-PCR analysis showed that the transcript level of Rc1 allele in white coleoptiles was clearly lower than in red coleoptiles, suggesting that Rc1 allele plays an important role in anthocyanin biosynthesis in coleoptiles of T. urartu. The allele isolation, phylogenetic analysis, geographical distribution and functional verification should be helpful in understanding the functions of Rc1 alleles and the molecular mechanism underlying the coleoptile color trait in Triticum.
机译：RC1（RC1（RED COReoptile）编码常见小麦的R2R3-MYB转录因子TamyB-A1，但尚未在Triticum urartu或Triticum Monocccum中报告同源基因，这两个种是古代基因组的来源。在这项研究中，在120吨Urartu accessions中发现了四个等位基因，以及来自9吨Monococcum access的两位等位基因。六个等位基因编码了五种蛋白质，因为TumbyB-A1-F和TumyB-A1-C编码了相同的蛋白质。这些等位基因的氨基酸序列中的所有突变位点都不在R2R3和转录活化剂结构域中，并且不影响保守的色氨酸（W）残基，也不影响MyB蛋白的“D / ELX2R / KX3LX3RX3R”基序。来自T. monocccum的等位基因与TamyB-B1和TamyB-D1更相似，而不是来自系统发育树中的T.Ureartu的TamyB-D1，而T.Urartu的等位基因比TMYB-A1更像T. monocccum等位基因。 TumyB-A1-C是分布式最广泛的等位基因，与地理分布分析中的其他五个国家相比，土耳其含有最大的遗传变异。在ZMR的帮助下，TumyB-A1-D或TumyB-A1-F或TumyB-A1-F诱导了小麦栽培品种“露珠”的白杨蛋白生物合成诱导的花青素生物合成。 RT-PCR分析表明，白色植物鞘细菌中RC1等位基因的转录水平明显低于红殖民石斛，表明RC1等位基因在乌雷鲁的植物科学生的植物原体中发挥着重要作用。等位基因分离，系统发育分析，地理分布和功能性验证应该有助于理解RC1等位基因的功能和小鼠植物色素特征的分子机制。

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