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首页> 外文期刊>Genome >Molecular analysis of anthocyanin biosynthesis-related genes reveal BoTT8 associated with purple hypocotyl of broccoli (Brassica oleracea var. italica L.)
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Molecular analysis of anthocyanin biosynthesis-related genes reveal BoTT8 associated with purple hypocotyl of broccoli (Brassica oleracea var. italica L.)

机译：花青素生物合成相关基因的分子分析显示与西兰花的紫色下丘脑相关的杆8（Brassica Oleracea Var。Italica L.）

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摘要

Broccoli (Brassica oleracea var. italica L.) is a highly nutritious vegetable that typically forms pure green or purple florets. However, green broccoli florets sometimes accumulate slight purplish pigmentation in response environmental factors, decreasing their market value. In the present study, we aimed to develop molecular markers to distinguish broccoli genotypes as pure green or purplish floret color at the early seedling stage. Anthocyanins are known to be involved in the purple pigmentation in plants. The purplish broccoli lines were shown to accumulate purple pigmentation in the hypocotyls of very young seedlings; therefore, the expression profiles of the structural and regulatory genes of anthocyanin biosynthesis were analyzed in the hypocotyls using qRT-PCR. BoPAL, BoDFR, BoMYB114, BoTT8, BoMYC1.1, BoMYC1.2, and BoTTG1 were identified as putative candidate genes responsible for the purple hypocotyl color. BoTT8 was much more highly expressed in the purple than green hypocotyls; therefore, it was cloned and sequenced from various broccoli lines, revealing SNP and InDel variations between these genotypes. We tested four SNPs (G > A; A > T; G > C; T > G) in the first three exons and a 14-bp InDel (ATATTTATATATAT) in the BoTT8 promoter in 51 broccoli genotypes, and we found these genetic variations could distinguish the green lines, purple lines, and F-1 hybrids. These novel molecular markers could be useful in broccoli breeding programs to develop a true green or purple broccoli cultivar.
机译：西兰花（Brassica Oleracea Var。Italica L.）是一种高营养的蔬菜，通常形成纯绿色或紫色小花。然而，绿色西兰花小花有时会积累轻微的紫色色素沉着，以响应环境因素，降低其市场价值。在本研究中，我们旨在开发分子标记，将西兰花基因型区分为早期幼苗阶段的纯绿色或紫色小花卉颜色。已知花青素参与植物中的紫色色素沉着。显示呈紫色的西兰花线，在非常年轻的幼苗的幼粒子中积累紫色色素沉积;因此，使用QRT-PCR分析在幼杆状的幼杆状中的结构和调节基因的表达谱。贝普尔，BODFR，BOMYB114，BOTT8，BOMYC1.1，BOMYC1.2和BOTTG1被鉴定为负责紫色缺点的推定候选基因。瓶子在紫色比绿色缺口更高度表达;因此，它被克隆并从各种西兰花线中测序，揭示了这些基因型之间的SNP和诱导变化。在51种Croccoli基因型中，在前三个外显子和14-BP Indel（Atatttatatatattat）中测试了四个SNP（g> a> t; g> g）。我们发现这些遗传变异可以区分绿线，紫线和F-1杂种。这些新的分子标记物可用于西兰花育种计划，以发展真正的绿色或紫色西兰花品种。

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