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DNA barcoding of species of Bacopa coupled with high-resolution melting analysis

机译：DNA条形码与高分辨率熔化分析相结合的贝加泊帕

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摘要

In Thailand, there are three species of Bacopa, namely, B. monnieri, B. caroliniana, and B. floribunda. Among these species of Bacopa, B. monnieri is the only medicinal species, used for the treatment of cognitive impairment and improvement of cognitive abilities because of its bioactive constituents, bacoside A and B. However, because of the similar characteristics of these species, it is difficult to differentiate among related species, resulting in confusion during identification. For this reason, and to ensure therapeutic quality for consumers, authentication is important. In this study, the three abovementioned species of Bacopa were evaluated using barcoding coupled with high-resolution melting (Bar-HRM) analysis based on primers designed for the trnL-F sequences of the three species. The melting profiles of the trnL-F amplicons of B. caroliniana and B. floribunda were clearly different from the melting profile of the trnL-F amplicon from B. monnieri; thus, the species could be discriminated by Bar-HRM analysis. Bar-HRM was then used to authenticate commercial products in various forms. The melting curves of the six commercial samples indicated that all the tested products contained genuine B. monnieri species. This method provides an efficient and reliable authentication system for future commercial herbal products and offers a reference system for quality control.
机译：在泰国，有三种Bacopa，即B. Monnieri，B. Caroliniana和B.Floribunda。在这些种类的Bacopa中，B. monnieri是唯一用于治疗认知障碍和认知能力的唯一药物，因为其生物活性成分，BacoSide A和B.然而，由于这些物种的类似特征，它难以区分相关的物种，导致在鉴定期间混淆。出于这个原因，为消费者确保治疗质量，认证很重要。在该研究中，使用基于为三种物种的TrNL-F序列设计的引物，使用条形码与高分辨率熔融（Bar-HRM）分析相结合来评估三种上述贝加帕。 B. Caroliniana和B.Floribunda的TRNL-F扩增子的熔化谱与来自B.Monnieri的Trnl-F扩增子的熔化谱不同;因此，可以通过Bar-HRM分析来区分该物种。然后，酒吧HRM用于以各种形式验证商业产品。六种商业样品的熔化曲线表明，所有测试产品含有真正的B.蒙尼埃。该方法为未来的商业草药产品提供了一种有效可靠的认证系统，并为质量控制提供了参考系统。

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