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首页> 外文期刊>Genomics >Integration of miRNA weighted gene co-expression network and miRNA-mRNA co-expression analyses reveals potential regulatory functions of miRNAs in calf rumen development
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Integration of miRNA weighted gene co-expression network and miRNA-mRNA co-expression analyses reveals potential regulatory functions of miRNAs in calf rumen development

机译：miRNA加权基因共表达网络和miRNA-mRNA共表达分析的整合揭示了小牛瘤胃发育中miRNA的潜在调节功能

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摘要

This study aimed to explore the roles of microRNAs (miRNAs) in calf rumen development during early life. Rumen tissues were collected from 16 calves (8 at pre-weaning and 8 at post-weaning) for miRNA-sequencing, differential expression (DE), miRNA weighted gene co-expression network (WGCNA) and miRNA-mRNA coexpression analyses. 295 miRNAs were identified. Bta-miR-143, miR-26a, miR-145 and miR-27b were the most abundantly expressed. 122 miRNAs were significantly DE between the pre- and post-weaning periods and the most up- and down-regulated miRNAs were bta-miR-29b and bta-miR-493, respectively. Enrichment analyses of the target genes of DE miRNAs revealed important roles for miRNA in rumen developmental processes, immune system development, protein digestion and processes related to the extracellular matrix. WGCNA indicated that bta-miR-145 and bta-miR-199a-3p are important hub miRNAs in the regulation of these processes. Therefore, bta-miR-143, miR-29b, miR-145, miR-493, miR-26a and miR-199 family members might be key regulators of calf rumen development during early life.
机译：本研究旨在探讨在早期生命期间小牛瘤胃发育的MicroRNAS（miRNA）的作用。从16个犊牛（在断奶后8例，在后断奶后8时）收集瘤胃组织，用于miRNA测序，差异表达（DE），miRNA加权基因共表达网络（WGCNA）和miRNA-mRNA共抑制分析。确定了295 miRNA。 BTA-MIR-143，MIR-26A，MIR-145和MIR-27B是最丰富的表达。在断奶后和断奶后期和断奶后期的122 miRNA显着下降，最高调节的miRNA分别是BTA-MIR-29B和BTA-MIR-493。 De miRNA的靶向基因的富集分析显示了瘤胃发育过程中miRNA的重要作用，免疫系统发育，蛋白质消化和与细胞外基质有关的过程。 WGCNA表明，BTA-MIR-145和BTA-MIR-19A-3P在这些过程的调节中是重要的枢纽MIRNA。因此，BTA-MIR-143，MIR-29B，MIR-145，MIR-493，MIR-26A和MIR-199家庭成员可能是早期生命期间小牛瘤胃发育的关键调节因素。

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