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Transcriptomic response of Drosophila melanogaster pupae developed in hypergravity

机译:德洛伐克果蝇的转录组反应

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Altered gravity can perturb normal development and induce corresponding changes in gene expression. Understanding this relationship between the physical environment and a biological response is important for NASA's space travel goals. We use RNA-Seq and qRT-PCR techniques to profile changes in early Drosophila melanogaster pupae exposed to chronic hypergravity (3 g, or three times Earth's gravity). During the pupal stage, D. melanogaster rely upon gravitational cues for proper development. Assessing gene expression changes in the pupae under altered gravity conditions helps highlight gravity-dependent genetic pathways. A robust transcriptional response was observed in hypergravity-treated pupae compared to controls, with 1513 genes showing a significant (q < 0.05) difference in gene expression. Five major biological processes were affected: ion transport, redox homeostasis, immune response, proteolysis, and cuticle development.
机译:重力改变可以扰动正常发育,并诱导基因表达的相应变化。 了解物理环境与生物反应之间的这种关系对于美国国家航空航天局的空间旅行目标是重要的。 我们使用RNA-SEQ和QRT-PCR技术在早期的果蝇Melanogaster Pupae暴露于慢性超高刻(3g或三次地球的重力)。 在蛹阶段,D. Melanogaster依赖引力线索进行适当的发展。 在改变的重力条件下评估蛹的基因表达变化有助于突出引力依赖性遗传途径。 与对照相比,在血液处理过的蛹中观察到鲁棒转录响应,其中1513个基因显示基因表达的显着(Q <0.05)差异。 五个主要生物过程受到影响:离子运输,氧化还原稳态,免疫应答,蛋白水解和角质层发育。

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