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Evaluation of collagenase gold plus BP protease in isolating islets from human pancreata

机译：胶原酶Gold Plus BP蛋白酶在分离人胰腺癌中的评价

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ABSTRACT: Selection of enzymes for optimal pancreas digestion is essential for successful human islet isolations. The aim of this study was to evaluate the efficacy and outcome of using Collagenase Gold plus BP protease (VitaCyte) (n = 8) by comparing it to two commercially available enzymes, Liberase MTF C/T (Roche) (n = 48) and Collagenase NB1/NP (Serva) (n = 15). The isolation outcomes were assessed by islet counting, viability, glucose-stimulated oxygen consumption rate (OCR), and successful graft-rate following transplantation in diabetic NOD scid mice. The pancreas donor characteristics were not significantly different between the tested enzyme groups regarding their BMI, pancreas weight, cold ischemia time (CIT) and HbA1c. The results show that digested tissue volume was not statistically significant between the VitaCyte enzyme (34.25 ± 5.4 mL) and the Roche enzyme (55.25 ± 3.42 mL, p = 0.073), however, this was significant with Serva enzyme (64.07 ± 7.95 mL, p = 0.020). Interestingly, the islet yields were not statistically different between all enzyme groups. Moreover, when islets were transplanted into NOD scid mice, the reversal rate of diabetes for the VitaCyte enzyme group was similar to all enzyme groups. In conclusion, the effectiveness of Collagenase Gold plus BP protease is comparable to the MTF C/T and the Collagenase NB1/NP enzymes; the low cost could facilitate the use of more pancreata for islet isolations. ? 2018 The Author(s). Published with license by Taylor & Francis ? 2018, ? Bashar Khiatah, Amber Tucker, Kuan-Tsen Chen, Rachel Perez, Shiela Bilbao, Luis Valiente, Leonard Medrano, Jeffrey Rawson, Elena Forouhar, Keiko Omori, Fouad Kandeel, Meirigeng Qi and Ismail H. Al-Abdullah.
机译：摘要：用于最佳胰腺消化的酶选择对于成功的人体胰岛分离是必不可少的。本研究的目的是通过将其与两种商业上可获得的酶进行比较，评估使用胶原酶Gold Plus BP蛋白酶（Vitacyte）（n = 8）的疗效和结果，使其酶MTF C / T（Roche）（n = 48）和胶原酶NB1 / NP（SERVA）（n = 15）。通过胰岛计数，活力，葡萄糖刺激的氧消耗率（OCR）评估分离结果，以及在糖尿病NOD SCID小鼠中移植后的成功接枝速率。在关于其BMI，胰腺重量，冷缺血时间（CIT）和HBA1C的测试酶基团之间的胰腺供体特性没有显着差异。结果表明，消化的组织体积在Vitacyte酶（34.25±5.4ml）和罗氏酶之间没有统计学意义（55.25±3.42ml，p = 0.073），这与伺服酶有显着性（64.07±7.95ml， p = 0.020）。有趣的是，所有酶组之间的胰岛产率在统计学上没有统计学。此外，当胰岛移植到点链小鼠中时，培体酶组糖尿病的逆转率类似于所有酶组。总之，胶原酶金加BP蛋白酶的有效性与MTF C / T和胶原酶NB1 / NP酶相当;低成本可能有助于使用更多的胰岛分离。还2018年作者。泰勒＆弗朗西斯发布的牌照？ 2018年，？ Bashar Khiatah，Amber Tucker，Kuan-Tsen Chen，Rachel Perez，Shiela Bilbao，Luis Valiente，Leonard Medrano，Jeffrey Rawson，Elena Forouhar，Keiko Omori，Fouad Kandeel，Meirigeng Qi和Ismail H. Al-Abdullah。

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