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首页> 外文期刊>Food & Function >Effects of the apple matrix on the postprandial bioavailability of flavan-3-ols and nutrigenomic response of apple polyphenols in minipigs challenged with a high fat meal
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Effects of the apple matrix on the postprandial bioavailability of flavan-3-ols and nutrigenomic response of apple polyphenols in minipigs challenged with a high fat meal

机译：苹果基质对苹果多酚苹果多酚的黄香兰-3-OLS和营养素反应的影响，用高脂粉挑战

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Food matrix interactions with polyphenols can affect their bioavailability and as a consequence may modulate their biological effects. The aim of this study was to determine if the matrix and its processing would modulate the bioavailability and the postprandial nutrigenomic response to a dietary inflammatory stress of apple flavan-3-ol monomers. We carried out an acute randomized controlled study in minipigs challenged with a high fat meal (HFM) supplemented with raw fruit, puree, or apple phenolic extract with matched content of flavan-3-ol monomers. Fasting and postprandial blood samples were collected over 3 h to quantify flavan-3-ol monomers in sera by UPLC-Q-TOF/MS and to isolate peripheral blood mononuclear cells (PBMCs) for assessing the changes in the gene expression profile using a microarray analysis. When compared to the extract-supplemented meal, the peak of the total flavan-3-ol concentration was reduced by half with both raw apple and puree supplements. The apple matrices also affected the gene expression profile as revealed by the Principal Component Analysis of the microarray data from PBMCs which discriminated the supplementation of HFM with the polyphenol extract from those with raw apples or puree. A total of 309 genes were identified as differentially expressed by the apple-derived products compared to HFM, with 63% modulated only in the presence of the food matrix (apple and puree). The number of differentially modulated genes was higher with the puree (246) than with the unprocessed apple (182). Pathway enrichment analyses revealed that genes affected by the apple-derived products control inflammation and leukocyte transendothelial migration both involved in the onset of atherosclerotic processes. Overall, this study showed that the two apple matrices reduce the postprandial serum concentration of flavon-3-ols whereas they increase the nutrigenomic response of PBMCs. The biological processes identified as modulated by the apple products suggest an attenuation of the transient pro-inflammatory response induced by a HFM. The differences observed between the nutrigenomic responses support that the apple matrix and its processing affect the nutrigenomic response, probably by increasing the bioavailability of other apple phytochemicals. To conclude, this study raises awareness for considering the impact of the food matrix and its processing on the biological response of polyphenols in nutritional studies.
机译：与多酚的食物基质相互作用可以影响其生物利用度，结果可以调节它们的生物学效应。本研究的目的是确定基质及其处理是否会调节生物利用度和对苹果黄山-3-醇单体膳食炎症应激的生物利用度和餐后营养素反应。我们对抗脂肪膳食（HFM）挑战的MINIPIG的急性随机对照研究进行了补充有生水果，泥炭或苹果酚类提取物，其具有匹配的香兰-3-醇单体含量。在3小时内收集禁食和餐后血液样品，通过UPLC-Q-TOF / MS在血清中量化Flavan-3-醇单体，并分离外周血单核细胞（PBMC）来使用微阵列评估基因表达谱的变化分析。与萃取液的膳食相比，总黄烷-3-醇浓度的峰值与原料苹果和泥质补充剂减少了一半。苹果基质还影响了基因表达谱，如PBMC的微阵列数据的主要成分分析所揭示的，这些分析由来自苹果或泥的多酚提取物的HFM的补充。与HFM相比，共鉴定了总体表达的309个基因，仅在食物基质（Apple和Puree）存在下63％调节63％。用未处理的苹果（182），仿形基因的数量高于纯块（246）。途径富集分析显示，受苹​​果衍生的产品控制炎症和白细胞转型迁移影响的基因，涉及动脉粥样硬化过程的发作。总体而言，这项研究表明，两种苹果矩阵减少了黄酮-3-Ols的餐后血清浓度，而它们增加了PBMC的营养学反应。苹果产品调节鉴定的生物学方法表明HFM诱导的瞬态促炎反应的衰减。营养素应答之间观察到的差异支持苹果基质及其加工影响营养素的反应，可能通过增加其他苹果植物化学物质的生物利用度。为了得出结论，本研究提高了对考虑食物基质的影响及其对营养研究中多酚生物反应的影响的认识。

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    《Food & Function》 |2020年第6期|共14页
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  • 正文语种 eng
  • 中图分类 食品工业;
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