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首页> 外文期刊>Fish & Shellfish Immunology >Scophthalmus maximus interleukin-1 beta limits Edwardsiella piscicida colonization in vivo
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Scophthalmus maximus interleukin-1 beta limits Edwardsiella piscicida colonization in vivo

机译：scophthalmus maximus白细胞介素-1β限制Edwardsiella Piscicida殖民化在体内

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摘要

Interleukine-1 beta (IL-1 beta) is the first identified pro-inflammatory cytokine, which is cleaved by caspase-1 following the inflammasomes activation, playing critical roles in innate immunity. However, few studies have been performed to characterize the IL-1 beta in lower vertebrates. Herein, we distinguished the Scophthalmus maximus IL-1 beta (SmIL-1 beta) from three like sequences and found that SmIL-1 beta was cleaved by S. maximus caspase at a non-conserved Asp(86), then targeted to the plasma membrane. Moreover, during the immersion infection of Edwardsiella piscicida, we found that E. piscicida were mainly colonized in gills at early time points and invaded to systemic sites after 5 days post infection, which was consistent with the dynamic up-regulated transcription of SmIL-1 beta. Furthermore, knockdown of SrnIL-1 beta promotes the bacterial colonization in gills at early time points and result into systemic colonization, while overexpression of SmIL-1 beta hampers the bacterial colonization in both spleen and kidney. Taken together, these data provide new insights into the molecular mechanisms of SmIL-1 beta and reveal its role in limiting bacterial infection in vivo, which will support the idea for better understanding the evolutionary of IL-1 beta functions in teleost.
机译：白细胞血荷脂-1β（IL-1β）是第一个鉴定的促炎细胞因子，其在发炎物激活后通过Caspase-1切割，在先天免疫中发挥关键作用。然而，已经进行了很少的研究以表征下脊椎动物中的IL-1β。在此，我们将scophthalmus maximus IL-1β（SMIL-1β）与三种类似的序列区分开，发现SMIL-1β在非保守的ASP（86）处由S.Maximus Caspase切割。然后靶向等离子体膜。此外，在Edwardsiella Piccicida的浸没感染期间，我们发现E.Piscicida在早期点的鳃中主要是在鳃中殖民，并在感染后5天后侵入全身位点，这与Smil-1的动态上调转录一致。 β。此外，SrnIL-1β的敲低在早期点促进鳃中的细菌定植，并导致全身定植，而SmIl-1β的过度表达妨碍了脾脏和肾脏中的细菌定植。这些数据携带，这些数据对Smil-1β的分子机制提供了新的见解，并揭示了其在限制体内细菌感染方面的作用，这将支持更好地了解IL-1β功能在Teactost中的进化。

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  • 来源
    《Fish & Shellfish Immunology》 |2019年第2019期|共10页
  • 作者

    Chen Shouwen; Ma Xin; Wu Di; Yang Dahai; Zhang Yuanxing; Liu Qin;

  • 作者单位

    East China Univ Sci &

    Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

    East China Univ Sci &

    Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

    East China Univ Sci &

    Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

    East China Univ Sci &

    Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

    East China Univ Sci &

    Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

    East China Univ Sci &

    Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

    Scophthabnus maximus; Interleukin-1 beta; Edwardsiella piscicida; Bacterial colonization;

    机译：scophthabnus maximus;白细胞介素-1 beta;Edwardsiella piscicida;细菌殖民化;

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