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首页> 外文期刊>Fish & Shellfish Immunology >pik3r3b, a novel immune-related gene in Nile tilapia (Oreochromis niloticus): Identification, expression and analysis of antibacterial activity
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pik3r3b, a novel immune-related gene in Nile tilapia (Oreochromis niloticus): Identification, expression and analysis of antibacterial activity

机译：Pik3R3B，一种新型免疫相关基因在尼罗罗非鱼（Oreochromis Niloticus）：抗菌活性的鉴定，表达和分析

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摘要

A full-length cDNA encoding phosphatidylinositol 3-kinase regulatory subunit gamma b gene in Nile tilapia (Oreochromis niloticus), termed as On-pik3r3b, was identified and characterized in this study. The sequence analysis demonstrated that the full-length cDNA of On-pik3r3b was 2018 bp, containing a 5' untranslated region (UTR) of 171 bp, an open reading frame (ORF) of 1422 bp and a 3' UTR of 425 bp. Its protein sequence displayed a high degree of identity with other fish. Using qPCR, the expression patterns of On-pik3r3b were investigated. In healthy Nile tilapia, the transcripts of On-pik3r3b were detected in all examined tissues, except the skin. Upon the stimulation with Streptococcus agalactiae, the On pik3r3b expression level in liver, spleen, kidney and gill were significantly increased at 12 h after infection. The recombinant On-pik3r3b showed in vitro antibacterial activity, against S. agalactiae and E. coli. Our observation strongly indicates that On-pik3r3b is involved in the innate immune response in Nile tilapia.
机译：在本研究中鉴定并表征了在尼罗替尼亚（Oreochromis NiLoticus）中编码磷脂酰肌醇3-激酶调节亚基γB基因的全长cDNA。序列分析证明，PIK3R3B的全长cDNA是2018年的BP，含有171bp的5'未翻转区域（UTR），1422bp的开放阅读框（ORF）和425bp的3'UTR。其蛋白质序列与其他鱼类显示出高度的身份。使用QPCR，研究了PIK3R3B的表达模式。在健康的尼罗基亚，除了皮肤外，在所有检查的组织中检测到pik3R3b的转录物。在用链球菌嗜血酰胺刺激后，感染后12小时在肝脏，脾脏，肾脏和鳃中的PIK3R3B表达水平显着增加。重组on-PIK3R3B显示出体外抗菌活性，对抗S.嗜虾和大肠杆菌。我们的观察强烈表明，PIK3R3B涉及尼罗罗非鱼的先天免疫应答。

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  • 来源
    《Fish & Shellfish Immunology》 |2019年第2019期|共9页
  • 作者

    Meng Liang; Xu Wen-teng; Chen Ya-dong; Wei Min; Cui Zhong-kai; Liu Yang; Guo Hua; Gan Xi; Zhu Jia-jie; Wang Lin-na; Chen Song-lin;

  • 作者单位

    Chinese Acad Fishery Sci Yellow Sea Fisheries Res Inst Minist Agr Key Lab Sustainable Dev Marine Fisheries Qingdao 266071 Shandong Peoples R China;

    Chinese Acad Fishery Sci Yellow Sea Fisheries Res Inst Minist Agr Key Lab Sustainable Dev Marine Fisheries Qingdao 266071 Shandong Peoples R China;

    Chinese Acad Fishery Sci Yellow Sea Fisheries Res Inst Minist Agr Key Lab Sustainable Dev Marine Fisheries Qingdao 266071 Shandong Peoples R China;

    Chinese Acad Fishery Sci Yellow Sea Fisheries Res Inst Minist Agr Key Lab Sustainable Dev Marine Fisheries Qingdao 266071 Shandong Peoples R China;

    Chinese Acad Fishery Sci Yellow Sea Fisheries Res Inst Minist Agr Key Lab Sustainable Dev Marine Fisheries Qingdao 266071 Shandong Peoples R China;

    Chinese Acad Fishery Sci Yellow Sea Fisheries Res Inst Minist Agr Key Lab Sustainable Dev Marine Fisheries Qingdao 266071 Shandong Peoples R China;

    Chinese Acad Fishery Sci Yellow Sea Fisheries Res Inst Minist Agr Key Lab Sustainable Dev Marine Fisheries Qingdao 266071 Shandong Peoples R China;

    Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

    Hlth Aquac Nanning 530021 Peoples R China;

    Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

    Hlth Aquac Nanning 530021 Peoples R China;

    Chinese Acad Fishery Sci Yellow Sea Fisheries Res Inst Minist Agr Key Lab Sustainable Dev Marine Fisheries Qingdao 266071 Shandong Peoples R China;

    Chinese Acad Fishery Sci Yellow Sea Fisheries Res Inst Minist Agr Key Lab Sustainable Dev Marine Fisheries Qingdao 266071 Shandong Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

    Phosphatidylinositol 3-kinase regulatory subunit gamma; Nile tilapia; Expression pattern; Immune response; Streptococcus agalactiae;

    机译：磷脂酰肌醇3-激酶调节亚基γ;尼罗拉亚岛;表达模式;免疫应答;链球菌嗜症症;

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