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首页> 外文期刊>Fish & Shellfish Immunology >ATG10 (autophagy-related 10) regulates the formation of autophagosome in the anti-virus immune response of pacific oyster (Crassostrea gigas)
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ATG10 (autophagy-related 10) regulates the formation of autophagosome in the anti-virus immune response of pacific oyster (Crassostrea gigas)

机译：ATG10（与自噬有关10）调节在太平洋牡蛎的抗病毒免疫应答中的自噬体（Crassostraea Gigas）的形成

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Autophagy, a highly conserved intracellular degradation system, is involved in numerous processes in vertebrate and invertebrate, such as cell survival, ageing, and immune responses. However, the detailed molecular mechanism of autophagy and its immune regulatory role in bivalves are still not well understood. In the present study, an autophagy-related protein ATG10 (designated as CgATG10) was identified from Pacific oyster Crassostrea gigas. The open reading frame of CgATG10 cDNA was of 621 bp, encoding a polypeptide of 206 amino acid residues with an Autophagy_act_C domain (from 96 to 123 amino acid), which shared high homology with that from C. virginica and Octopus bimaculoides. The mRNA transcripts of CgATG10 were widely expressed in all the tested tissues including mantle, gonad, gills, hemocytes and hepatopancreas, with the highest expression level in mantle. After the stimulation with poly (I:C), the mRNA expression level of CgATG10 in the mantle of oysters was significantly up-regulated (4.92-fold of that in Blank group, p 0.05), and the LC3-conversion from LC3-I to LC3-II (LC3-II/LC3-0 also increased. After an additional injection of dsRNA to knock-down the expression of CgATG10 (0.33-fold and 0.10-fold compared respectively with Blank group and dsGFP group, p 0.05), the downstream conversion of CgLC3 was inhibited significantly compared with that of the control dsGFP group, while the expression level of autophagy-initiator CgBeclin1 did not change significantly. In addition, the mRNA transcripts of interferon regulatory factor CgIRF-1 increased significantly in CgATG10-knockdown oysters at 12 h post poly (I:C) stimulation. All the results indicated that CgATG10 might participate in the immune response against poly (I:C) by regulating autophagosome formation and interferon system in oysters.
机译：Autophagy是一种高度保守的细胞内降解系统，涉及脊椎动物和无脊椎动物的许多方法，例如细胞存活，老化和免疫应答。然而，自噬的详细分子机制及其免疫调节作用仍然无法清楚地理解。在本研究中，从太平洋牡蛎鲫鱼鉴定了自噬相关蛋白ATG10（指定为CGATG10）。 CGATG10 cDNA的开放阅读框为621bp，用自噬棒_癌域（从96-123个氨基酸）编码206个氨基酸残基的多肽，其与来自C. Virginica和八达通血管素的高同源性。 CGATG10的mRNA转录物在所有测试组织中被广泛表达，包括披肩，性腺，GONAD，鳃，血细胞和肝癌，在披风中的表达水平最高。用聚（I：C）刺激后，牡蛎壳体中CgATG10的mRNA表达水平显着上调（在空白组中4.92倍，P <0.05），以及来自LC3的LC3-转化率-I至LC3-II（LC3-II / LC3-0也增加。额外注射DSRNA以敲击CGATG10的表达（分别与空白组和DSGFP组相比的0.33倍和0.10倍，p＆lt <0.05），与对照DSGFP组的CG13的下游转化显着抑制，而自噬 - 引发剂CGBeclin1的表达水平没有显着变化。此外，干扰素调节因子CGIRF-1的mRNA转录物显着增加在CGATG10-opotholdown Oysters，在聚（i：c）刺激后12小时。所有结果表明，CGATG10可以通过调节牡蛎中的自噬体形成和干扰素系统来参与对聚（I：C）的免疫应答。

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