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首页> 外文期刊>Fish & Shellfish Immunology >Identification and characterization of differentially expressed genes in hepatopancreas of oriental river prawn Macrobrachium nipponense under nitrite stress
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Identification and characterization of differentially expressed genes in hepatopancreas of oriental river prawn Macrobrachium nipponense under nitrite stress

机译：亚硝酸盐应激下东方河虾大鼠嗜酸线肝癌肝癌中差异表达基因的鉴定与表征

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摘要

The oriental river prawn Macrobrachium nipponense is a highly adaptable, tolerant, and fecund freshwater prawn that inhabits a wide range of aquatic environments. The hepatopancreas of crustaceans is not only a site for secretion of digestive enzymes, and also plays important roles in several metabolic processes, such as lipid and carbohydrate metabolism. It is the main organ for the detoxification and immunity. In this study, high-throughput sequencing techniques were used to detect the effect of nitrite stress (10 mg/L nitrite-N for 48 h) on gene expression in the hepatopancreas of M. nipponense. A total of 13,769 million reads were harvested, and 94,534 transcripts were de novo assembled using Trinity software and produced 56,054 non-redundant transcripts. A total of 825 differentially expressed genes were obtained comparing 48 h nitrite stress with control group. In the analysis of GO and KEGG database, significant differences were found in 49 pathways. Immune-related pathways under nitrite stress included arginine and proline metabolism, glutamate metabolism, Jak-Stat signaling pathway, endocytosis, wnt signaling pathway, RIG-I-like receptor signaling pathway, TGF-beta signaling pathway, GnRH signaling pathway and phagosome. Apoptosis-related pathway was also significantly altered, such as lysosome and apoptosis. Remarkably, nitrite stress altered the expression patterns of key apoptosis genes (tetraspanins-like protein, LAMP, CD63, caspase 3C and Caspase 1) and immune genes (Serine proteinase-like protein, C-type lectin, daf-36, SOCS-2, alpha-2-macroglobulin), confirmed that nitrite-stress induce immune response and eventually even apoptosis. This study provided a new insight into the role of hepatopancreas in crustaceans, and further investigation will continue.
机译：东方河虾大虾长崎Nippoonense是一种高度适应，宽容，和腓特水虾，栖息着各种水生环境。甲壳类动物的肝癌不仅是消化酶分泌的部位，而且还在几种代谢过程中起重要作用，例如脂质和碳水化合物代谢。它是解毒和免疫力的主要器官。在该研究中，使用高通量测序技术来检测亚硝酸盐应激（10mg / L Nitrite-n持续48小时）对M. nippoonense的肝癌中的基因表达的影响。收获了13,769万读，94,534份成绩单是使用Trinity软件组装的De Novo，并产生了56,054个非冗余成绩单。将总共​​825个差异表达基因与对照组进行比较48小时亚硝酸盐胁迫。在Go和Kegg数据库的分析中，在49条途径中发现了显着的差异。亚硝酸盐应激下的免疫相关途径包括精氨酸和脯氨酸代谢，谷氨酸代谢，jak-stat信号传导途径，内吞作用，信号传导途径，钻石-i样受体信号通路，TGF-β信号通路，GNRH信号通路和吞噬症。凋亡相关的途径也显着改变，例如溶酶体和凋亡。值得注意的是，亚硝酸盐应激改变了关键凋亡基因的表达模式（四苯胺状蛋白，灯，CD63，Caspase 3C和Caspase 1）和免疫基因（丝氨酸蛋白样蛋白，C型凝集素，DAF-36，SOC-2 ，α-2-甲基蛋白）证实亚硝酸盐胁迫诱导免疫应答，最终甚至凋亡。本研究为肝癌在甲壳类动物中的作用提供了新的洞察力，并继续进行进一步调查。

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  • 来源
    《Fish & Shellfish Immunology》 |2019年第2019期|共11页
  • 作者

    Yu Jielun; Ji Xiangshan; Wang Xuepeng; Li Tongming; Wang Hui; Zeng Qifan;

  • 作者单位

    Shandong Agr Univ Coll Anim Sci &

    Technol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Anim Sci &

    Technol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Anim Sci &

    Technol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Anim Sci &

    Technol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Anim Sci &

    Technol Tai An 271018 Shandong Peoples R China;

    Auburn Univ Program Cell &

    Mol Biosci Sch Fisheries Aquaculture &

    Aquat Sci Fish Mol Genet &

    Biotechnol Lab Auburn AL 36849 USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

    Macrobrachium nipponense; Nitrite; Transcriptome; Apoptosis; Immune response;

    机译：Macrobarium nippoonense;亚硝酸盐;转录组;细胞凋亡;免疫应答;

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