...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Fish & Shellfish Immunology >Effects of cortisol and lipopolysaccharide on expression of select growth-, stress- and immune-related genes in rainbow trout liver
【24h】

Effects of cortisol and lipopolysaccharide on expression of select growth-, stress- and immune-related genes in rainbow trout liver

机译：皮质醇和脂多糖对虹鳟鱼选择生长，胁迫和免疫相关基因表达的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Many studies have shown that stress-induced cortisol levels negatively influence growth and immunity in finfish. Despite this knowledge, few studies have assessed the direct effects of cortisol on liver immune function. Using real-time PCR, the expression of three cortisol-responsive genes (GR: glucocorticoid receptor, IGF-1: insulin-like growth factor-I and SOCS-1: suppressor of cytokine signaling-I), genes involved with innate and adaptive immunity (IL-1 beta: interleukin-1 beta, IgM: immunoglobin-M and Lyz: lysozyme), and liver-specific antimicrobial peptides (hepcidin and LEAP-2A: liver-expressed antimicrobial peptide-2A) was studied in vitro using rainbow trout liver slices. The abundances of GR, SOCS-1 and IGF-1 mRNAs were suppressed by cortisol treatment. Abundance of IL-1 beta mRNA was upregulated by LPS and suppressed by cortisol treatment in a time-dependent manner. While abundance of IgM mRNA was suppressed by cortisol treatment and stimulated by LPS, there were no effects of cortisol or LPS on abundance of Lyz mRNA. Abundance of hepcidin and LEAP-2A mRNA levels were suppressed by cortisol treatment and stimulated by LPS. These results demonstrate that cortisol directly suppresses abundance of GR, IGF-1, IL-1 beta, IgM, hepcidin, LEAP-2A and SOCS-1 mRNA transcripts in the rainbow trout liver. We report for the first time, a suppressive effect of cortisol (within 8 h of treatment) on hepcidin and LEAP-2A mRNAs in rainbow trout liver, which suggests that acute stress may negatively affect liver immune function in rainbow trout.
机译：许多研究表明，应激诱导的皮质醇水平对唾液中的生长和免疫产生负面影响。尽管有这些知识，但很少有研究已经评估了皮质醇对肝免疫功能的直接影响。使用实时PCR，表达三种皮质响应基因（GR：糖皮质激素受体，IGF-1：胰岛素样生长因子-I和SOCS-1：细胞因子信令-I的抑制剂，与先天和适应性涉及的基因免疫（IL-1β：白细胞介素-1β，IgM：Immunoglobin-M和Lyz：溶菌酶）和肝脏特异性抗微生物肽（Hepcidin和Leap-2a：肝脏表达的抗微生物肽-2a）进行了使用彩虹研究鳟鱼肝切片。通过皮质醇治疗抑制了GR，SOC-1和IGF-1 mRNA的丰度。通过LPS上调IL-1βmRNA的丰度，并以时间依赖的方式通过皮质醇处理抑制。虽然通过皮质醇处理抑制了丰度的IgM mRNA并受到LPS刺激的虽然通过LPS刺激，但在莱斯mRNA的丰度上没有皮质醇或LPS的影响。通过皮质醇处理抑制了肝素和LEAP-2A mRNA水平的丰度，并受LPS刺激。这些结果表明，皮质醇直接抑制了虹鳟鱼中的GR，IGF-1，IL-1β，IgM，肝素，LEAP-2A和SOCS-1 mRNA转录物。我们首次报告，皮质素（治疗8小时内）对肝素和摇滚鳟鱼肝脏的肺炎素和Leap-2a mRNA的抑制作用，这表明急性胁迫可能会对虹鳟鱼的肝脏免疫功能产生负面影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号