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首页> 外文期刊>Fish & Shellfish Immunology >The immunoprotective effect of whole-cell lysed inactivated vaccine with SWCNT as a carrier against Aeromonas hydrophila infection in grass carp
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The immunoprotective effect of whole-cell lysed inactivated vaccine with SWCNT as a carrier against Aeromonas hydrophila infection in grass carp

机译：全细胞裂解的灭活疫苗的免疫保护作用与SWCNT作为草鲤鱼患有AeroMonas疏水液感染的载体

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摘要

Aeromonas hydrophila is a strong gram-negative bacterium that can cause a mass death of grass carp, and result in the huge economic loss. Development of practical vaccines is the best way to control the outbreak of this bacterial disease. In this study, a whole-cell inactivated vaccine was obtained via sonication, and then single-walled carbon nanotubes (SWCNTs) was used to link to the bacterial lysate (BL) for a novel vaccine (SWCNTs-BL). A total of 400 fish were vaccinated with BL and SWCNTs-BL via immersion (5, 10 mg L-1) or injection (5, 10 mu g/fish) before challenge with live A. hydrophila at the 28 days post immunization (d.p.i.). The results showed that the antibody titer, enzymatic activity, expression of some immune-related genes (especially IgM and TNF-alpha) and RPS of fish in the injection groups were significantly increased compared to the control group after 28 d.p.i. For the immersion groups, immunological parameters were increased compared to the control group. Furthermore, the immuno-protective effects of SWCNTs-BL were better than BL. The above results indicated that BL of A. hydrophila can effectively induce specific immune response of grass carp, and BL linked with functionalized SWCNTs could enhance the protective effect of immersion immunization. Our results may provide a practical vaccine, with a simple production, to fight against bacterial diseases in aquaculture industry.
机译：Aeromonas疏水杆菌是一种强烈的革兰阴性细菌，可以引起草鲤鱼的大规模死亡，导致经济损失巨大。实际疫苗的发展是控制这种细菌疾病爆发的最佳方式。在该研究中，通过超声处理获得全细胞灭活疫苗，然后使用单壁碳纳米管（SWCNTs）连接到新型疫苗（SWCNTS-B1）的细菌裂解物（BL）中。通过浸渍（5,10mg L-1）或注射（5,10μg/鱼），在免疫后28天在28天攻击（DPI ）。结果表明，在28d.p.i之后，与对照组相比，抗体滴度，酶活性，一些免疫相关基因的表达（特别是IgM和TNF-α）和RPS的鱼类的鱼类的鱼类显着增加。对于浸渍基团，与对照组相比，免疫参数增加。此外，SWCNTS-B1的免疫保护作用优于BL。上述结果表明，A.疏水物的BL可以有效地诱导草鲤鱼的特定免疫应答，并且与官能化SWCNTS连接的BL可以增强浸没免疫的保护作用。我们的结果可以提供一种实用的疫苗，具有简单的生产，以防止水产养殖业中的细菌疾病。

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  • 来源
    《Fish & Shellfish Immunology》 |2020年第2020期|共8页
  • 作者

    Zhang Zhongyu; Liu Gaoyang; Ma Rui; Qi Xiaozhou; Wang Gaoxue; Zhu Bin; Ling Fei;

  • 作者单位

    Northwest A&

    F Univ Coll Anim Sci &

    Technol Xinong Rd 22nd Yangling 712100 Shaanxi Peoples R China;

    Northwest A&

    F Univ Coll Anim Sci &

    Technol Xinong Rd 22nd Yangling 712100 Shaanxi Peoples R China;

    Northwest A&

    F Univ Coll Anim Sci &

    Technol Xinong Rd 22nd Yangling 712100 Shaanxi Peoples R China;

    Northwest A&

    F Univ Coll Anim Sci &

    Technol Xinong Rd 22nd Yangling 712100 Shaanxi Peoples R China;

    Northwest A&

    F Univ Coll Anim Sci &

    Technol Xinong Rd 22nd Yangling 712100 Shaanxi Peoples R China;

    Northwest A&

    F Univ Coll Anim Sci &

    Technol Xinong Rd 22nd Yangling 712100 Shaanxi Peoples R China;

    Northwest A&

    F Univ Coll Anim Sci &

    Technol Xinong Rd 22nd Yangling 712100 Shaanxi Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

    Aeromonas hydrophila; Single-walled carbon nanotubes; Grass carp; Immune response; Inactivated vaccine;

    机译：Aeromonas疏水龙;单壁碳纳米管;草鲤;免疫应答;灭活疫苗;

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