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首页> 外文期刊>Fish & Shellfish Immunology >Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila
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Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila

机译：时间分辨的RNA-SEQ为患有Aeromonas疏水菌感染后，对欧洲鳗鱼（Anguilla Anguilla）的肠免疫反应进行了新的认识

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摘要

No studies systematically examined the intestinal immune response for yellow stage of European eel (Anguilla anguilla) with Aeromonas hydrophila infection by time-resolved RNA-seq. Here, we examined transcriptional profiles of the intestines at three-time points following infection with A. hydrophila. Intraperitoneal injections caused mortalities within 48 h post-injection (hpi), with the survival rate 87.5% at 24 hpi and 83.9% at 48 hpi. The result from KEGG pathway enrichment analysis showed that the immune related "cytosolic DNA-sensing pathway" was significantly enriched at the first and second time points (6 hpi and 18 hpi), with the upregulated expression of irf3, ill b, tnfaip3, cxcl8a, ap1-2, c-fos, polr3d, polr3g and polr3k both at 6 hpi and 18 hpi, but not at the third time point (36 hpi). According to the KEGG annotation, 326 immune and inflammation-related DEGs were found. The co-expression network of those 326 DEGs revealed the existence of three modules, and tlrl was found to be in the center of the biggest module which contained massive DEGs from "signal transduction" and "transport and catabolism". The c3 isoforms showed different expression pattern among the three time points, indicating a unique activation of complement systems at 18 hpi. Furthermore, two cathelicidins (aaCATH_1 and aaCATH_2) were highly up-regulated at the first two time points, and the bacterial growth inhibition assay revealed their antibacterial properties against A. hydrophila. Our data indicated the important roles of cytosolic DNA-sensing pathway, as well as transcripts including tlr1, c3, polr and cathelicidins in the intestine of A. anguilla in response to A. hydrophila infection. The present study will provide leads for functional studies of host-pathogen interactions.
机译：没有研究通过时间分辨的RNA-SEQ对欧洲鳗鱼（Anguilla Anguilla）黄色阶段的肠道免疫反应进行了系统地检查了肠道免疫反应。在这里，我们在用A.疏水杆菌感染后，检查了在感染后的三次肠道的转录谱。腹膜内注射导致注射后48小时内的死亡率（HPI），其存活率为87.5％，24 HPI，48 HPI为83.9％。 Kegg途径富集分析结果表明，在第一和第二时间点（6 HPI和18 HPI）下显着富集了免疫相关的“细胞溶质DNA感测途径”，其IRF3，ILL B，TNFAIP3，CXCL8A的上调表达，AP1-2，C-FOS，POLR3D，POLR3G和POLR3K在6 HPI和18 HPI，但不是在第三个时间点（36 HPI）。根据Kegg注释，发现326个免疫和炎症相关的次数。那些326°的共表达网络揭示了三个模块的存在，并且发现TLRL在最大模块的中心中，其中包含来自“信号转导”和“传输和分解代谢”的大规模降低。 C3同种型在三个时间点中显示出不同的表达模式，表明18 HPI的补体系统的独特激活。此外，在前两个时间点高度上调两种水癌（AAcath_1和Aacath_2），细菌生长抑制测定显示它们对A.疏水物的抗菌性能。我们的数据表明了细胞溶质DNA传感途径的重要作用，以及包括在A.Ingophila感染的A. Anguilla的肠道中的转录物。本研究将提供宿主病原体相互作用的功能研究的引用。

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  • 来源
    《Fish & Shellfish Immunology》 |2020年第1期|共13页
  • 作者

    Xiong Fan; Xiong Jing; Wu Ya Fang; Cao Lu; Huang Wen Shu; Chang Ming Xian;

  • 作者单位

    Chinese Acad Sci Inst Hydrobiol State Key Lab Freshwater Ecol &

    Biotechnol Key Lab Aquaculture Dis Control Wuhan 430072 Hubei Peoples R China;

    Jimei Univ Engn Res Ctr Modern Technol Eel Ind Minist Educ PR China Xiamen 361021 Peoples R China;

    Jimei Univ Engn Res Ctr Modern Technol Eel Ind Minist Educ PR China Xiamen 361021 Peoples R China;

    Chinese Acad Sci Inst Hydrobiol State Key Lab Freshwater Ecol &

    Biotechnol Key Lab Aquaculture Dis Control Wuhan 430072 Hubei Peoples R China;

    Jimei Univ Engn Res Ctr Modern Technol Eel Ind Minist Educ PR China Xiamen 361021 Peoples R China;

    Chinese Acad Sci Inst Hydrobiol State Key Lab Freshwater Ecol &

    Biotechnol Key Lab Aquaculture Dis Control Wuhan 430072 Hubei Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

    Transcriptome analysis; European eel; Aeromonas hydrophila; Cytosolic DNA-Sensing pathway; Cathelicidins; Intestine;

    机译：转录组分析;欧洲鳗鱼;Aeromonas疏水液;细胞源DNA传感途径;肠霉素;肠道;

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