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Suitability of total nitrogen as a predictor of protein content in stream periphyton

机译：总氮的适用性是珀西氏菌中蛋白质含量的预测因子

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Despite its importance in trophic dynamics, the protein content of food resources is often estimated using N-to-protein conversion factors. This method assumes that all N is contained in proteins and that all proteins are 16% N by mass. However, if the environmental factors affecting protein content differ from those affecting N content, conflating protein and N contents could lead to a misunderstanding of foodweb dynamics. We directly measured the protein content of periphyton collected from 46 streams in southeastern New York State (USA) using a modified Lowry method and compared it to the protein content of the same periphyton estimated with the traditional 6.25 mg protein/mg N conversion factor. We assessed the potential ecological drivers of protein and N contents based on correlations between these 2 metrics and various environmental factors. Directly measured protein content was positively correlated with N content, but mean directly measured protein content was <1/2 of mean estimated protein content. The mean N:protein of the periphyton in our study was 2.47 +/- 1.10 (SD), less than all applicable published conversion factors, which ranged from 4.44 to 6.48. In addition, protein content was correlated positively with canopy cover, whereas N content was correlated positively with P content. Our results indicate that N content is a poor predictor of protein content and that the protein and N contents of periphyton may have different ecological drivers. Considering the protein and N contents of basal food resources separately may allow greater understanding of the role of nutritional quality in foodweb dynamics.
机译：尽管其在营养性动态方面重要性，但仍使用N-蛋白转化因子估计食物资源的蛋白质含量。该方法假设蛋白质中的所有n包含在蛋白质中，并且所有蛋白质均为16％N质量。然而，如果影响蛋白质含量的环境因素与影响N含量的那些不同，则融合蛋白质和N内容可能导致对食品生态的误解。我们使用改性的洛数法测量了从东南部的纽约州（美国）的46个溪流中收集的Periphyton的蛋白质含量，并将其与传统的6.25mg蛋白/ mg N转化系数相比估计的相同植物红的蛋白质含量。我们根据这2个度量和各种环境因素之间的相关性评估了蛋白质和N个内容的潜在生态驱动因素。直接测量的蛋白质含量与N含量呈正相关，但是平均直接测量的蛋白质含量为<1/2的平均估计蛋白质含量。我们研究中Periphyton的平均值为2.47 +/- 1.10（SD），低于所有适用的公布转换因子，范围为4.44至6.48。另外，蛋白质含量与冠层盖相关，而N含量与P含量正相关。我们的结果表明，N含量是蛋白质含量差的差，蛋白质和斑纹的含量可能具有不同的生态驱动因素。考虑蛋白质和基础食品资源的N个目的，可以更加了解营养质量在食品培养动态中的作用。

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