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首页> 外文期刊>Fish Physiology and Biochemistry >Expressions and characterization of MuRFs, Atrogin-1, F-box25 genes in tilapia, Oreochromis niloticus, in response to starvation
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Expressions and characterization of MuRFs, Atrogin-1, F-box25 genes in tilapia, Oreochromis niloticus, in response to starvation

机译：Murfs，Atrogin-1，Tilapia，Oreochromis niloticus的F-Box25基因的表达和表征，响应饥饿

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Muscle accretion is affected by the difference between protein synthesis and its degradation. Studies on different species revealed that muscle proteolysis is mediated by different pathways including the ubiquitin-proteasome pathway in which the ubiquitin protein ligases play an important role. These muscle atrophy associated ligases were not well studied in tilapia. In this study, we characterized the ubiquitin protein ligases MuRF1/2/3, Atrogin-1 and F-box25, members of the ubiquitin-proteasome pathway in tilapia, Oreochromis niloticus, and their expressions in the muscle of starved, fed, refed, and control fish. Sequences of these genes revealed presence of Ring finger, B-box, and Cos domains in all MuRF genes, as well as F-box domain in Atrogin-1 and F-box25 genes. Real-time qPCR data analysis showed that expression of MuRF1/2/3, Atrogin-1, F-box25, and proteasome complex genes was significantly upregulated in starved fish compared to fed fish. Concurrently, the proteasome activity was 1.7-folds elevated in the starved fish compared to fed fish. These results confirm the important role of these genes in muscle degradation and suggest potential usage as markers of muscle accretion in tilapia.
机译：肌肉含量受蛋白质合成的差异及其降解的影响。不同物种的研究表明，肌肉蛋白水解是由不同途径介导的，包括泛素蛋白质角质酶起到重要作用的泛素 - 蛋白酶体途径。这些肌肉萎缩的相关性酶在罗非鱼没有很好地研究过。在这项研究中，我们以泛素-1和F-Box25，乌柏蛋白 - 蛋白酶体途径的成员，葡萄球菌蛋白蛋白质途径的成员及其在饥饿，喂养，喂养的肌肉中的表达，和控制鱼。这些基因的序列显示出在所有Murf基因中的无名指，B盒和COS结构域的存在，以及亚胚素-1和F-Box25基因中的F盒结构域。实时QPCR数据分析表明，与喂鱼相比，Murf1 / 2/3，rolf1 / 2/3，atrogin-1，F栏25和蛋白酶体复杂基因的表达显着上调。与Fed鱼相比，同时，在饥饿的鱼中，蛋白酶体活性为1.7倍。这些结果证实了这些基因在肌肉降解中的重要作用，并表明潜在的用法作为罗非鱼肌肉吸收的标志物。

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