...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function >Molecular characterization and expression analyses of cDNAs encoding the thioredoxin-interacting protein and selenoprotein P genes and histological changes in Nile tilapia (Oreochromis niloticus) in response to silver nanoparticle exposure
【24h】

Molecular characterization and expression analyses of cDNAs encoding the thioredoxin-interacting protein and selenoprotein P genes and histological changes in Nile tilapia (Oreochromis niloticus) in response to silver nanoparticle exposure

机译：接触银纳米颗粒的硫氧还蛋白相互作用蛋白和硒蛋白P基因编码cDNA的分子表征和表达分析以及尼罗罗非鱼（Oreochromis niloticus）的组织学变化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Herein, Nile tilapia thioredoxin-interacting protein (On-TXNIP) and selenoprotein P (On-SEPP) cDNAs were cloned and characterized. The full-length On-TXNIP cDNA contained 2 arrestin domains, 2 conserved cysteine residues that bind to thioredoxin to inhibit thioredoxin function, and 2 PPXY motifs, which negatively regulate the protein by stimulating binding to E3 ubiquitin ligase. The On-SEPP cDNA contained 17 selenocysteines (Sec) encoded by the TGA codon, which can be recognized as either a stop codon or a Sec codon. The On-SEPP cDNA also carried 2 typical SECIS elements located in the 3'UTR that are important for selenocysteine translation. Evolutionary analyses of both the On-TXNIP and On-SEPP genes revealed that these genes are closely related to the TXNIP and SEPP genes in zebrafish (Danio redo), with amino acid similarities of 91.8% and 61.9%, respectively. A normal tissue distribution analysis indicated that the On-TXNIP and On-SEPP genes were ubiquitously expressed in all tissues examined, and the highest expression levels of these genes were observed in peripheral blood leukocytes (PBLs) and the trunk kidney, respectively. The expression levels of On-TXNIP and On-SEPP transcripts were acutely and chronically analyzed following the injection of fish with 1,10 or 100 mg/kg silver nano particles (Ag NPs). Significant up-regulation of On-TXNIP and On-SEPP transcripts was observed in the liver, spleen, and head kidney at the early phase of Ag NP exposure (hours 6 through 48). Down-regulation of On-SEPP transcripts was clearly observed in the liver at weeks 1 to 4. Histopathology analysis demonstrated that the fish livers exhibited a dramatic infiltration of Kupffer cells, elevated bi-nucleated cells, expanded sinusoidal blood congestion and severe necrosis in a dose-dependent manner. Based on these findings, coupling of the expression analysis of these two cellular stress response genes and histopathological observation of fish exposed to Ag NPs should be reliable for the assessment of Ag NP contamination in teleost fish. (C) 2015 Elsevier B.V. All rights reserved.
机译：在此，克隆和鉴定了尼罗罗非鱼硫氧还蛋白相互作用蛋白（On-TXNIP）和硒蛋白P（On-SEPP）cDNA。全长On-TXNIP cDNA包含2个抑制蛋白结构域，2个与硫氧还蛋白结合以抑制硫氧还蛋白功能的保守半胱氨酸残基和2个PPXY基序，它们通过刺激与E3泛素连接酶的结合来负调控蛋白质。 On-SEPP cDNA包含17个由TGA密码子编码的硒代半胱氨酸（Sec），可以将其识别为终止密码子或Sec密码子。 On-SEPP cDNA还带有位于3'UTR中的2个典型SECIS元件，这些元件对于硒代半胱氨酸翻译很重要。对On-TXNIP和On-SEPP基因的进化分析表明，这些基因与斑马鱼（Danio redo）中的TXNIP和SEPP基因密切相关，氨基酸相似性分别为91.8％和61.9％。正常组织分布分析表明，On-TXNIP和On-SEPP基因在所有检查的组织中普遍表达，并且这些基因的最高表达水平分别在外周血白细胞（PBLs）和躯干肾脏中观察到。在向鱼类注射1,10或100 mg / kg银纳米颗粒（Ag NPs）后，急性和长期分析On-TXNIP和On-SEPP转录本的表达水平。在Ag NP暴露的早期（第6至48小时），在肝脏，脾脏和头部肾脏中观察到On-TXNIP和On-SEPP转录显着上调。在第1至4周时，肝脏中明显观察到On-SEPP转录物的下调。组织病理学分析表明，鱼肝中Kupffer细胞大量浸润，双核细胞增多，正弦血流扩大和严重坏死。剂量依赖性。基于这些发现，这两个细胞应激反应基因的表达分析与暴露于Ag NPs的鱼的组织病理学观察相结合，对于评估硬骨鱼中的Ag NP污染应该是可靠的。 （C）2015 Elsevier B.V.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号